Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering.
Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering.
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DOI：
10.1016/j.biomaterials.2013.05.066
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发表时间：
2013-09
期刊：
影响因子：
14
通讯作者：
Banerjee, Ipsita
中科院分区：
文献类型：
--
作者：
Goh, Saik-Kia;Bertera, Suzanne;Olsen, Phillip;Candiello, Joseph E.;Halfter, Willi;Uechi, Guy;Balasubramani, Manimalha;Johnson, Scott A.;Sicari, Brian M.;Kollar, Elizabeth;Badylak, Stephen F.;Banerjee, Ipsita
关键词：
全球约有2.85亿人患有糖尿病，胰岛素补充是最常见的治疗措施。再生医学方法，如生物工程胰腺，已被提议作为潜在的治疗替代方案。生物工程胰腺将受益于生物支架的开发，这种支架可支持并增强细胞功能和组织发育。灌注脱细胞器官很可能是此类支架的候选材料，因为它们模拟天然器官的组成、结构和生物力学性质。在这项研究中，我们研究了整个胰腺的灌注脱细胞以及用胰腺细胞类型对整个胰腺支架进行再细胞化的可行性。我们的结果表明，整个胰腺的灌注脱细胞可有效去除细胞和核物质，同时保留具有可灌注血管和导管网络以及关键细胞外基质（ECM）成分的复杂三维微观结构。为了模拟胰腺细胞组成，我们用腺泡细胞系和β细胞系对整个胰腺支架进行再细胞化，并培养长达5天。我们的结果显示，在脱细胞胰腺内细胞成功植入，并且所得到的移植物使胰岛素基因表达强烈上调。这些发现支持整个胰腺细胞外基质作为生物材料支架在支持和增强胰腺细胞功能方面的生物学效用，并代表了利用再生医学方法迈向生物工程胰腺的一步。
Approximately 285 million people worldwide suffer from diabetes, with insulin supplementation as the most common treatment measure. Regenerative medicine approaches such as a bioengineered pancreas has been proposed as potential therapeutic alternatives. A bioengineered pancreas will benefit from the development of a bioscaffold that supports and enhances cellular function and tissue development. Perfusion-decellularized organs are a likely candidate for use in such scaffolds since they mimic compositional, architectural and biomechanical nature of a native organ. In this study, we investigate perfusion-decellularization of whole pancreas and the feasibility to recellularize the whole pancreas scaffold with pancreatic cell types. Our result demonstrates that perfusion-decellularization of whole pancreas effectively removes cellular and nuclear material while retaining intricate three-dimensional microarchitecture with perfusable vasculature and ductal network and crucial extracellular matrix (ECM) components. To mimic pancreatic cell composition, we recellularized the whole pancreas scaffold with acinar and beta cell lines and cultured up to 5 days. Our result shows successful cellular engraftment within the decellularized pancreas, and the resulting graft gave rise to strong up-regulation of insulin gene expression. These findings support biological utility of whole pancreas ECM as a biomaterials scaffold for supporting and enhancing pancreatic cell functionality and represent a step toward bioengineered pancreas using regenerative medicine approaches.
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影响因子：
14
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
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Badylak, Stephen F.
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通讯作者：
Bloomgarden, ZT
影响因子：
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作者：
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