Cloning of the structural gene for Clostridium botulinum type C1 toxin and whole nucleotide sequence of its light chain component
Cloning of the structural gene for Clostridium botulinum type C1 toxin and whole nucleotide sequence of its light chain component
复制标题
肉毒梭菌C1型毒素结构基因的克隆及其轻链组分全核苷酸序列
DOI：
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复制
发表时间：
1991
影响因子：
4.4
通讯作者：
K. Oguma
中科院分区：
文献类型：
--
作者：
K. Kimura;N. Fujii;K. Tsuzuki;T. Murakami;T. Indoh;N. Yokosawa;K. Oguma
C1型肉毒梭菌的致炎性由特异性噬菌体介导。DNA是从其中一个样本中提取的。用λ gt 11噬菌体和大肠杆菌克隆了能产生与抗毒素血清反应的蛋白质的两个DNA片段，大小分别为3 kb和7.8kb。然后将两个DNA片段亚克隆到pUC 118质粒中，并转移到E.大肠杆菌细胞。用双脱氧链终止法对克隆的DNA片段进行核苷酸序列分析，并用Western免疫印迹法对基因产物进行分析。7.8-kb片段编码毒素的整个轻链组分和重链组分的N末端，而3-kb片段编码剩余的重链组分。测定轻链组分的整个核苷酸序列，并将衍生的氨基酸序列与破伤风毒素的氨基酸序列进行比较。结果发现，C1毒素的轻链组分具有几个氨基酸区域，除了N末端，这是同源的破伤风毒素。
The toxigenicity of Clostridium botulinum type C1 is mediated by specific bacteriophages. DNA was extracted from one of these phages. Two DNA fragments, 3 and 7.8 kb, which produced the protein reacting with antitoxin serum were cloned by using bacteriophage lambda gt11 and Escherichia coli. Both DNA fragments were then subcloned into pUC118 plasmids and transferred into E. coli cells. The nucleotide sequences of the cloned DNA fragments were analyzed by the dideoxy chain termination method, and their gene products were analyzed by Western immunoblot. The 7.8-kb fragment coded for the entire light chain component and the N terminus of the heavy chain component of the toxin, whereas the 3-kb fragment coded for the remaining heavy chain component. The entire nucleotide sequence for the light chain component was determined, and the derived amino acid sequence was compared with that of tetanus toxin. It was found that the light chain component of C1 toxin possessed several amino acid regions, in addition to the N terminus, that were homologous to tetanus toxin.
B 型和 E 型肉毒杆菌神经毒素的部分氨基酸序列。
影响因子：
3.9
作者：
Schmidt,JJ;Sathyamoorthy,V;DasGupta,BR
通讯作者：
DasGupta,BR
A型肉毒杆菌神经毒素重链和轻链的部分氨基酸序列。
DOI：
10.1016/0006-291x(84)90858-1
发表时间：
1984
影响因子：
3.1
作者：
Schmidt,JJ;Sathyamoorthy,V;DasGupta,BR
通讯作者：
DasGupta,BR