Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte.
Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte.
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DOI：
10.1021/nn101638u
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发表时间：
2010-09-28
期刊：
影响因子：
17.1
通讯作者：
Liang XJ
中科院分区：
文献类型：
--
作者：
Guo S;Huang Y;Jiang Q;Sun Y;Deng L;Liang Z;Du Q;Xing J;Zhao Y;Wang PC;Dong A;Liang XJ
利用层层法制备的电荷反转功能金纳米粒子，将小干扰RNA(SiRNA)和质粒DNA导入癌细胞。聚丙烯酰胺凝胶电泳法对siRNA的检测证实了功能金纳米粒子的电荷反转特性。利用电荷反转功能金纳米颗粒辅助转染增强型绿色荧光蛋白(EGFP)，提高了EGFP的表达效率，同时对细胞增殖的毒性也大大降低。电荷反转功能金纳米粒子携带的核膜蛋白Lamin A/C-siRNA能有效地沉默核膜蛋白Lamin A/C，其敲除效率优于商品化的脂质体2000。激光共聚焦扫描显微镜图像显示，花菁5-siRNA/聚乙烯亚胺/顺式乌头酸化poly(allylamine)/polyethyleneimine/11-mercaptoundecanoic酸金纳米粒子(cy5-siRNA/PEI/PAH-Cit/PEI/MUA-AuNP)络合物的胞质内分布着更多的cy5-siRNA。这些结果证明了利用电荷反转功能金纳米颗粒作为提高核酸输送效率的一种手段的可行性。
Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. The expression efficiency of enhanced green fluorescent protein (EGFP) was improved by adjuvant transfection with charge-reversal functional gold nanoparticles, which also had much lower toxicity to cell proliferation. Lamin A/C, an important nuclear envelope protein, was effectively silenced by lamin A/C-siRNA delivered by charge-reversal functional gold nanoparticles, whose knockdown efficiency was better than that of commercial Lipofectamine 2000. Confocal laser scanning microscopic images indicated that there was more cy5-siRNA distributed throughout the cytoplasm for cyanine 5-siRNA/polyethyleneimine/cis-aconitic anhydride-functionalized poly(allylamine)/polyethyleneimine/11-mercaptoundecanoic acid-gold nanoparticle (cy5-siRNA/PEI/PAH-Cit/PEI/MUA-AuNP) complexes. These results demonstrate the feasibility of using charge-reversal functional gold nanoparticles as a means of improving the nucleic acid delivery efficiency.
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影响因子：
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