Plasticity in expression of the glutamate transporters GLT-1 and GLAST in spinal dorsal horn glial cells following partial sciatic nerve ligation.
Plasticity in expression of the glutamate transporters GLT-1 and GLAST in spinal dorsal horn glial cells following partial sciatic nerve ligation.
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部分坐骨神经结扎后，谷氨酸转运蛋白表达的可塑性和脊柱背角胶质细胞中的胶状。
DOI：
10.1186/1744-8069-5-15
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发表时间：
2009-03-26
期刊：
影响因子：
3.3
通讯作者：
Dougherty PM
中科院分区：
文献类型：
--
作者：
Xin WJ;Weng HR;Dougherty PM
谷氨酸转运体清除突触释放的谷氨酸，从而终止谷氨酸能神经传递，尤其是位于星形胶质细胞中的谷氨酸转运体-1(GLT-1)和谷氨酸-天冬氨酸转运体(GLAST)。人们越来越清楚地认识到，GLT-1和GLAST在不同的生理和病理条件下会发生功能和表达的变化。本文用免疫组织化学方法研究了部分坐骨神经结扎(PSNL)后大鼠脊髓背角GLT-1和GLAST表达的可塑性。动物在PSNL后7天被证实对机械刺激产生超敏反应。在幼稚动物中，GLT-1和GLAST的基线表达只在星形胶质细胞中观察到，而在小胶质细胞和神经元中都没有观察到。小胶质细胞和星形胶质细胞在PSNL后7天和14天分别以OX-42和GFAP的表达增加为证据，显示出对神经损伤的反应。而GLT-1和GLAST蛋白的总水平在术后7天和14天均下降。重要的是，GLT-1和GLAST的细胞位置也因神经损伤而改变。而活化的星形胶质细胞GLT-1和GLAST的表达明显减少，活化的小胶质细胞在PSNL后7天开始表达GLT-1和GLAST，并维持到第14天。这些结果表明，神经损伤后，星形胶质细胞和小胶质细胞中谷氨酸转运体的表达受到不同程度的调节。
Clearance of synaptically released glutamate, and hence termination of glutamatergic neurotransmission, is carried out by glutamate transporters, most especially glutamate transporter-1 (GLT-1) and the glutamate-aspartate transporter (GLAST) that are located in astrocytes. It is becoming increasingly well appreciated that changes in the function and expression of GLT-1 and GLAST occur under different physiological and pathological conditions. Here we investigated the plasticity in expression of GLT-1 and GLAST in the spinal dorsal horn using immunohistochemistry following partial sciatic nerve ligation (PSNL) in rats. Animals were confirmed to develop hypersensitivity to mechanical stimulation by 7 days following PSNL. Baseline expression of GLT-1 and GLAST in naive animals was only observed in astrocytes and not in either microglia or neurons. Microglia and astrocytes showed evidence of reactivity to the nerve injury when assessed at 7 and 14 days following PSNL evidenced by increased expression of OX-42 and GFAP, respectively. In contrast, the total level of GLT-1 and GLAST protein decreased at both 7 and 14 days after PSNL. Importantly, the cellular location of GLT-1 and GLAST was also altered in response to nerve injury. Whereas activated astrocytes showed a marked decrease in expression of GLT-1 and GLAST, activated microglia showed de novo expression of GLT-1 and GLAST at 7 days after PSNL and this was maintained through day 14. Neurons showed no expression of GLT-1 or GLAST at any time point. These results indicate that the expression of glutamate transporters in astrocytes and microglia are differentially regulated following nerve injury.
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GLT-1的基因转移是神经胶质谷氨酸转运蛋白，通过重组腺病毒衰减大鼠的炎症和神经性疼痛，将其转移到脊髓中。
影响因子：
3.3
作者：
Maeda S;Kawamoto A;Yatani Y;Shirakawa H;Nakagawa T;Kaneko S
通讯作者：
Kaneko S
影响因子：
7.4
作者：
Ma, WY;Quirion, R
通讯作者：
Quirion, R
影响因子：
7.4
作者：
SELTZER, Z;DUBNER, R;SHIR, Y
通讯作者：
SHIR, Y
影响因子：
7.4
作者：
Liaw, WJ;Stephens, RL;Tao, YX
通讯作者：
Tao, YX
影响因子：
2.9
作者：
Cata, Juan P.;Weng, Han-Rong;Dougherty, Patrick M.
通讯作者：
Dougherty, Patrick M.