Combined oropharyngeal/nasal swab is equivalent to nasopharyngeal sampling for SARS-CoV-2 diagnostic PCR.
Combined oropharyngeal/nasal swab is equivalent to nasopharyngeal sampling for SARS-CoV-2 diagnostic PCR.
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DOI：
10.1186/s12866-021-02087-4
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发表时间：
2021-01-22
期刊：
影响因子：
4.2
通讯作者：
Verhasselt B
中科院分区：
文献类型：
--
作者：
Desmet T;Paepe P;Boelens J;Coorevits L;Padalko E;Vandendriessche S;Leroux-Roels I;Aerssens A;Callens S;Braeckel EV;Malfait T;Vermassen F;Verhasselt B
2020 年初，COVID-19 疫情成为国际关注的突发公共卫生事件。为了应对这一流行病，采用简单、舒适和可靠的测试方法进行广泛的测试是最重要的。鼻咽 (NP) 拭子采样是参考方法，但受到国际供应短缺的阻碍。使用更容易获得的咽拭子研究了一种新的口咽/鼻 (OP/N) 采样方法。 35 名患者通过 NP 或 OP/N 采样被诊断为 SARS-CoV-2。 31 名患者的配对拭子均呈阳性。一名入院时 NP 和 OP/N 拭子检测均为阴性的患者，最终通过支气管肺泡灌洗液确诊。配对拭子的病毒 RNA 载量之间发现了很强的相关性 (r = 0.76；P < 0.05)。住院患者（n = 28）中NP和OP/N分析的敏感性分别为89.3%和92.7%。本研究证明了通过 rRT-PCR 检测 SARS-CoV-2 时 NP 和 OP/N 采样的等效性。住院患者中 NP 和 OP/N 采样的敏感性都非常高。
Early 2020, a COVID-19 epidemic became a public health emergency of international concern. To address this pandemic broad testing with an easy, comfortable and reliable testing method is of utmost concern. Nasopharyngeal (NP) swab sampling is the reference method though hampered by international supply shortages. A new oropharyngeal/nasal (OP/N) sampling method was investigated using the more readily available throat swab. 35 patients were diagnosed with SARS-CoV-2 by means of either NP or OP/N sampling. The paired swabs were both positive in 31 patients. The one patient who tested negative on both NP and OP/N swab on admission, was ultimately diagnosed on bronchoalveolar lavage fluid. A strong correlation was found between the viral RNA loads of the paired swabs (r = 0.76; P < 0.05). The sensitivity of NP and OP/N analysis in hospitalized patients (n = 28) was 89.3% and 92.7% respectively. This study demonstrates equivalence of NP and OP/N sampling for detection of SARS-CoV-2 by means of rRT-PCR. Sensitivity of both NP and OP/N sampling is very high in hospitalized patients.
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