A Novel Ex vivo Culture Model for Inflammatory Bone Destruction
A Novel Ex vivo Culture Model for Inflammatory Bone Destruction
复制标题
一种新型的炎症性骨破坏离体培养模型
DOI：
10.1177/0022034513495240
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发表时间：
2013-07
影响因子：
7.6
通讯作者：
Waddington, R. J.
中科院分区：
文献类型：
--
作者：
Sloan, A. J.;Taylor, S. Y.;Smith, E. L.;Roberts, J. L.;Chen, L.;Wei, X. Q.;Waddington, R. J.
骨代谢平衡的病理改变是炎症性骨疾病如牙周病进展的核心。我们开发了一种新型的离体小鼠下颌骨炎症性骨破坏模型。将下颌骨切片在存在或不存在牙龈卟啉单胞菌脂多糖（LPS）或促炎细胞因子的情况下培养14天。培养后，通过定量基质蛋白、常驻破骨细胞、韧带细胞、单核细胞、巨噬细胞和中性粒细胞评估细胞活力和组织形态计量学。在没有炎症因子的情况下，培养物的活力，破骨细胞和基质成分得以维持。LPS或TNFα刺激显示细胞增殖、单核细胞、破骨细胞分化和基质降解增加。尽管不存在体循环，但可通过暴露于LPS和模型中TNFα的直接影响诱导病理生理性骨代谢，为炎性骨破坏和研究新型治疗方法的作用提供了模型。
Pathological alterations in the balance of bone metabolism are central to the progression of inflammatory bone diseases such as periodontal disease. We have developed and characterized a novel ex vivo murine mandible model of inflammatory bone destruction. Slices of mandible were cultured for 14 days in the presence or absence of P. gingivalis lipopolysaccharide (LPS) or pro-inflammatory cytokines. Following culture, cell viability and tissue histomorphometry were assessed with quantification of matrix proteins, resident osteoclasts, ligament cells, monocytes, macrophages, and neutrophils. In the absence of inflammatory factors, culture viability, osteoclasts, and matrix components were maintained. LPS or TNFα stimulation demonstrated an increase in cellular proliferation, monocyte cells, osteoclast differentiation, and matrix degradation. Pathophysiological bone metabolism can be induced via exposure to LPS and direct influence of TNFα within the model despite the absence of systemic circulation, providing a model for inflammatory bone destruction and investigation of the effects of novel therapeutics.
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影响因子：
5.4
作者：
Chen, Li;Wei, Xiao-Qing;Aeschlimann, Daniel
通讯作者：
Aeschlimann, Daniel
DOI：
10.1196/annals.1346.013
发表时间：
2006-01-01
期刊：
SKELETAL DEVELOPMENT AND REMODELING IN HEALTH, DISEASE, AND AGING
影响因子：
--
作者：
Roodman, G. David
通讯作者：
Roodman, G. David
巨噬细胞刺激因子刺激分离的破骨细胞中的存活和趋化行为。
DOI：
10.1084/jem.178.5.1733
发表时间：
1993-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fuller K;Owens JM;Jagger CJ;Wilson A;Moss R;Chambers TJ
通讯作者：
Chambers TJ
影响因子：
4.4
作者：
J. Owens;A. Gallagher;T. Chambers
通讯作者：
J. Owens;A. Gallagher;T. Chambers
影响因子：
3
作者：
Mogi, M;Otogoto, J;Kojima, K
通讯作者：
Kojima, K