Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization.
Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization.
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DOI：
10.1111/nph.13373
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Gilmartin PM
中科院分区：
文献类型：
--
作者：
Li J;Webster MA;Wright J;Cocker JM;Smith MC;Badakshi F;Heslop-Harrison P;Gilmartin PM
报春花的异形花发育受S基因控制。在针形花和穗形花中，控制花药位置、雌蕊长度和花粉大小的S位点基因尚未被鉴定。我们已经整合了S连锁基因，标记序列和突变表型，以创建P. vulgaris S位点区域的图谱，这将有助于识别关键的S位点基因。我们已经产生，测序和注释BAC序列跨越S基因座，并确定其染色体位置。我们采用了经典遗传学和三点杂交与重组体的分子遗传分析相结合的方法来生成图谱。我们已经通过Illumina测序和生物信息学分析以及染色体原位杂交对该区域进行了表征。我们提出了一个综合的遗传和物理图谱，在P. vulgaris S位点两侧的表型和DNA序列标记。BAC重叠群包含1.5 Mb的基因组区域，其中1 Mb的序列含有锚定到重叠BAC上的82个S连锁基因。S基因座位于最大的中部着丝粒染色体对的着丝粒附近。这些数据将有助于识别报春花中协调异型柱的基因，并能够对S基因座进行进化分析。
Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S‐linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three‐point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5‐Mb genomic region with 1 Mb of sequence containing 82 S‐linked genes anchored to overlapping BACs. The S locus is located close to the centromere of the largest metacentric chromosome pair. These data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus.
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影响因子：
2.9
作者：
Bridges, CB
通讯作者：
Bridges, CB
影响因子：
64.5
作者：
BHATTACHARYYA, MK;SMITH, AM;MARTIN, C
通讯作者：
MARTIN, C
DOI：
10.1007/bf01848862
发表时间：
1936-01-01
期刊：
ZEITSCHR INDUKT AB STAMM U VERERBUNGSL
影响因子：
--
作者：
ERNST, ALFRED
通讯作者：
ERNST, ALFRED
DOI：
10.1111/nph.13370
发表时间：
2015-10
期刊：
The New phytologist
影响因子：
--
作者：
Cocker JM;Webster MA;Li J;Wright J;Kaithakottil G;Swarbreck D;Gilmartin PM
通讯作者：
Gilmartin PM
DOI：
10.1098/rspb.1905.0049
发表时间：
1905-11-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-CONTAINING PAPERS OF A BIOLOGICAL CHARACTER
影响因子：
--
作者：
Bateson, W;Gregory, RP
通讯作者：
Gregory, RP