Injectable polyHIPEs as high-porosity bone grafts.
Injectable polyHIPEs as high-porosity bone grafts.
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DOI：
10.1021/bm2008839
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发表时间：
2011-10-10
影响因子：
6.2
通讯作者：
Cosgriff-Hernandez E
中科院分区：
文献类型：
--
作者：
Moglia RS;Holm JL;Sears NA;Wilson CJ;Harrison DM;Cosgriff-Hernandez E
高内相乳液（polyHIPE）的聚合是用于生产高孔隙率支架的相对较新的方法。这些聚HIPE泡沫的可调结构使其成为组织工程骨移植物的有吸引力的候选者。以前研究的聚HIPE系统需要有毒稀释剂或高固化温度，这禁止其用作可注射骨移植物。相比之下，我们已经开发了一种可注射的聚HIPE，其在生理温度下固化为刚性的高孔隙率泡沫。首先，合成了一种可生物降解的大分子单体，富马酸丙二醇酯二甲基丙烯酸酯（PFDMA），具有适当的粘度和疏水性乳化。表面活性剂的选择过程中的详细说明，特别关注的关键结构特征的聚合物（日志P值，氢键受体网站）和表面活性剂（HLB值，氢键供体网站），使稳定的HIPE形成。在37°C下孵育HIPE用于引发甲基丙烯酸酯基团的不饱和双键的自由基交联以使连续相稳定并锁定乳液几何形状。所得聚HIPE的孔隙率约为75%，孔径范围为4 - 29 μm，平均压缩模量和强度分别为33和5 MPa。这些发现突出了这些支架作为可注射的组织工程骨移植物的巨大潜力。
Polymerization of high internal phase emulsions (polyHIPEs) is a relatively new method for the production of high porosity scaffolds. The tunable architecture of these polyHIPE foams make them attractive candidates for tissue engineered bone grafts. Previously studied polyHIPE systems require either toxic diluents or high cure temperatures which prohibit their use as an injectable bone graft. In contrast, we have developed an injectable polyHIPE that cures at physiological temperatures to a rigid, high-porosity foam. First, a biodegradable macromer, propylene fumarate dimethacrylate (PFDMA), was synthesized that has appropriate viscosity and hydrophobicity for emulsification. The process of surfactant selection is detailed with particular focus on the key structural features of both polymer (log P values, hydrogen bond acceptor sites) and surfactant (HLB values, hydrogen bond donor sites) that enable stable HIPE formation. Incubation of HIPEs at 37°C was used to initiate radical crosslinking of the unsaturated double bond of the methacrylate groups to polymerize the continuous phase and lock in the emulsion geometry. The resulting polyHIPEs exhibited ~75% porosity, pore sizes ranging from 4 to 29 μm, and an average compressive modulus and strength of 33 and 5 MPa, respectively. These findings highlight the great potential of these scaffolds as injectable, tissue engineered bone grafts.
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影响因子：
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作者：
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通讯作者：
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