Blood oxygenation level-dependent (BOLD) functional MRI of visual stimulation in the rat retina at 11.7 T.
Blood oxygenation level-dependent (BOLD) functional MRI of visual stimulation in the rat retina at 11.7 T.
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DOI：
10.1002/nbm.1571
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发表时间：
2011-02
影响因子：
2.9
通讯作者：
Duong, Timothy Q.
中科院分区：
文献类型：
--
作者：
De La Garza, Bryan H.;Muir, Eric R.;Li, Guang;Shih, Yen-Yu I.;Duong, Timothy Q.
关键词：
虽然基于光学的成像技术提供了有价值的视网膜功能和生理信息，但它们大多限于视网膜表面的探测，并且需要无障碍的光路。相比之下，MRI可以提供没有深度限制的生理和功能数据。然而，薄大鼠视网膜的血氧水平依赖性功能性MRI（BOLD fMRI）是具有挑战性的，因为需要高空间分辨率，以及眼动和敏感性伪影的潜在存在。本研究报告了高分辨率（111 × 111 × 1000 µm3）BOLD fMRI在11.7T下麻醉大鼠视网膜视觉刺激的新应用。高场MRI扫描仪用于提高信噪比、空间分辨率和BOLD灵敏度。视觉刺激（8Hz漫射消色差光）强烈增加视网膜中的BOLD响应[5.0 ± 0.8%来自激活像素，3.1 ± 1.1%来自感兴趣的整个视网膜区域（平均值± SD），n = 6只大鼠的12次试验，与基线相比p < 0.05]。由于对视觉刺激的调节反射，在瞳孔和睫状肌周围检测到一些激活的像素，阿托品和苯巴比妥滴眼液可使其减少。没有视觉刺激的BOLD fMRI扫描显示没有显著激活的像素（全视网膜BOLD变化为0.08 ± 0.34%，n = 6次试验，5只大鼠，与基线无统计学差异，p > 0.05）。BOLD功能磁共振成像的视觉刺激有可能提供临床相关的数据来探测血流动力学神经血管耦合和视网膜功能障碍的深度分辨率。
Although optically based imaging techniques provide valuable functional and physiological information of the retina, they are mostly limited to the probing of the retinal surface and require an unobstructed light path. MRI, in contrast, could offer physiological and functional data without depth limitation. Blood oxygenation level-dependent functional MRI (BOLD fMRI) of the thin rat retina is, however, challenging because of the need for high spatial resolution, and the potential presence of eye movement and susceptibility artifacts. This study reports a novel application of high-resolution (111 × 111 × 1000 µm3) BOLD fMRI of visual stimulation in the anesthetized rat retina at 11.7 T. A high-field MRI scanner was utilized to improve the signal-to-noise ratio, spatial resolution and BOLD sensitivity. Visual stimuli (8 Hz diffuse achromatic light) robustly increased BOLD responses in the retina [5.0 ± 0.8% from activated pixels and 3.1 ± 1.1% from the whole-retina region of interest (mean ± SD), n = 12 trials on six rats, p < 0.05 compared with baseline]. Some activated pixels were detected surrounding the pupil and ciliary muscle because of accommodation reflex to visual stimuli, and were reduced with atropine and phenylephrine eye drops. BOLD fMRI scans without visual stimulations showed no significantly activated pixels (whole-retina BOLD changes were 0.08 ± 0.34%, n = 6 trials on five rats, not statistically different from baseline, p > 0.05). BOLD fMRI of visual stimulation has the potential to provide clinically relevant data to probe hemodynamic neurovascular coupling and dysfunction of the retina with depth resolution.
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影响因子：
3.8
作者：
Cheng H;Yan Y;Duong TQ
通讯作者：
Duong TQ
影响因子：
4.4
作者：
Li, Yingxia;Cheng, Haiying;Duong, Timothy Q.
通讯作者：
Duong, Timothy Q.
DOI：
10.1097/01.wcb.0000054755.93668.20
发表时间：
2003-04-01
影响因子：
6.3
作者：
Sicard, K;Shen, Q;Duong, TQ
通讯作者：
Duong, TQ
影响因子：
5.7
作者：
Sicard, KM;Duong, TQ
通讯作者：
Duong, TQ
影响因子：
5.3
作者：
Metea, MR;Newman, EA
通讯作者：
Newman, EA