Mating type influences chromosome loss and replicative senescence in telomerase-deficient budding yeast by Dnl4-dependent telomere fusion.
Mating type influences chromosome loss and replicative senescence in telomerase-deficient budding yeast by Dnl4-dependent telomere fusion.
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DOI：
10.1111/j.1365-2958.2008.06353.x
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发表时间：
2008-09
影响因子：
3.6
通讯作者：
Bailis AM
中科院分区：
文献类型：
--
作者：
Meyer DH;Bailis AM
随着年龄的增长，我们的大多数细胞逐渐丧失分裂能力，因为复制衰老是由于无法复制染色体末端而导致的。衰老的时间取决于端粒 DNA 的长度，当端粒 DNA 非常短时，会引发检查点信号。极短的端粒也容易受到有害重排、末端降解和端粒-端粒融合的影响。在这里，我们报告了非同源末端连接（NHEJ）的新作用，这是一种双链断裂（DSB）修复途径，影响端粒酶缺陷的酿酒酵母复制衰老动力学和染色体丢失率。在端粒酶缺陷的细胞中，NHEJ 的缺失会延迟复制衰老，减少衰老过程中活力的丧失，并抑制衰老相关的染色体丢失和端粒-端粒融合。单倍体和二倍体细胞中交配型基因表达的差异影响 NHEJ 功能，导致复制衰老的不同动力学。这些结果表明，单倍体和二倍体端粒酶缺陷型酵母复制衰老动力学的差异是由 NHEJ 依赖性端粒融合的变化决定的，可能是通过断裂-融合-桥 (BFB) 循环的启动。
As we age, the majority of our cells gradually lose the capacity to divide because of replicative senescence that results from the inability to replicate the ends of chromosomes. The timing of senescence is dependent on the length of telomeric DNA, which elicits a checkpoint signal when critically short. Critically short telomeres also become vulnerable to deleterious rearrangements, end degradation and telomere-telomere fusions. Here we report a novel role of non-homologous end joining (NHEJ), a pathway of double-strand break (DSB) repair in influencing both the kinetics of replicative senescence and the rate of chromosome loss in telomerase-deficient Saccharomyces cerevisiae. In telomerase-deficient cells, the absence of NHEJ delays replicative senescence, decreases loss of viability during senescence, and suppresses senescence-associated chromosome loss and telomere-telomere fusion. Differences in mating-type gene expression in haploid and diploid cells affect NHEJ function, resulting in distinct kinetics of replicative senescence. These results suggest that the differences in the kinetics of replicative senescence in haploid and diploid telomerase-deficient yeast is determined by changes in NHEJ-dependent telomere fusion, perhaps through the initiation of the breakage-fusion-bridge (BFB) cycle.
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DOI：
10.1007/bf00695392
发表时间：
1977-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
GOLIN, JE;ESPOSITO, MS
通讯作者：
ESPOSITO, MS
影响因子：
11.4
作者：
Espejel, S;Franco, S;Blasco, MA
通讯作者：
Blasco, MA
影响因子：
64.5
作者：
Hackett, JA;Feldser, DM;Greider, CW
通讯作者：
Greider, CW
影响因子：
14.9
作者：
Heacock ML;Idol RA;Friesner JD;Britt AB;Shippen DE
通讯作者：
Shippen DE
影响因子：
3.3
作者：
Carter, Sidney D.;Iyer, Shilpa;Astrom, Stefan U.
通讯作者：
Astrom, Stefan U.