Fast and reliable micro-bioassay technique based on biospeckle
Fast and reliable micro-bioassay technique based on biospeckle
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基于生物斑点的快速可靠的微生物检测技术
DOI：
10.1117/12.2651049
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Rajagopalan Uma Maheswari
中科院分区：
文献类型：
--
作者：
Devi Arti;Kadono Hirofumi;Rajagopalan Uma Maheswari
近年来，各种危险化学品造成的污染已成为一个非常严重的问题。目前，登记的化学物质有2亿种，从技术上和成本上来说，很难甚至不可能单独分析、识别每一种化学品，然后评估它们对环境的毒性。另一方面，生物测定已经得到了很多关注，其中基于微生物如浮游生物的反应评估环境毒性的毒性，而不单独识别每种化学品。对于该技术，需要显微镜观察以获得关键特征，例如活/死状态和游泳能力。对于较小的微生物，由于成像系统的焦深较窄，显微镜观察变得更加困难。在我们的研究中，为了克服这些困难，我们提出了一种新的技术，利用激光生物散斑的衍射场产生的浮游生物的微生物测定。将大小为30-40 µm的草履虫chilomonas暴露于不同的pH条件下，从对照7.2逐渐降低0.5至3.7。结果揭示了激光生物散斑在检测游泳行为的细微变化，微生物的健康与pH值变化方面的灵敏度，这表明了快速评估环境毒性的潜力。
Recently, the pollution caused by various hazardous chemicals has become a very serious problem. Currently, 200 million kinds of chemical substances are registered, and it is technically and costly very difficult or even impossible to analyse, identify each chemical individually and then evaluate their toxicity on the environment. On the other hand, bioassay has been getting a lot of attention where the toxicity of environmental toxicity is assessed based on the reaction of micro-organisms such as plankton without identifying each chemical individually. For this technique, a microscope observation is required to obtain critical features such as alive/dead status and swimming ability. With smaller microorganisms, microscope observation becomes more difficult due to the narrower focal depth of the imaging system. In our study, to overcome these difficulties, we proposed a novel technique for the micro-bioassay utilizing laser biospeckle in the diffraction field generated from plankton. Paramecium chilomonas of size 30-40 µm was exposed to different pH conditions from control 7.2 to gradually decreasing by 0.5 till 3.7. Results reveal the sensitivity of laser biospeckles in detecting the subtle changes in the swimming behavior, the health of the microbe with change in pH suggesting the potential for fast assessment the toxicity of an environment.
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pH值对裸藻和奇洛单胞菌向前游动的影响
DOI：
10.1086/physzool.27.3.30152169
发表时间：
1954
期刊：
Physiological Zoology
影响因子：
--
作者：
J. W. Lee
通讯作者：
J. W. Lee
DOI：
10.1177/0973408215600602b
发表时间：
2015-09
期刊：
Journal of Education for Sustainable Development
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.6
作者：
Shunmugam AP;Subramanian G;Fernandez JG
通讯作者：
Fernandez JG
使用尾草履虫进行急性毒性生物测定，草履虫是研究久效磷对游泳行为、形态和繁殖影响的关键成员
影响因子：
1.8
作者：
J. Venkateswara Rao;V. Gunda;K. Srikanth;S. Arepalli
通讯作者：
S. Arepalli
pH 对草履虫食物泡形成的影响
DOI：
10.1086/physzool.15.4.30151659
发表时间：
1942
期刊：
Physiological Zoology
影响因子：
--
作者：
IN Paramecium
通讯作者：
IN Paramecium