Three-dimensional micro-electrode array for recording dissociated neuronal cultures.
Three-dimensional micro-electrode array for recording dissociated neuronal cultures.
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DOI：
10.1039/b820596e
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发表时间：
2009-07-21
期刊：
影响因子：
6.1
通讯作者：
Wheeler BC
中科院分区：
文献类型：
--
作者：
Musick K;Khatami D;Wheeler BC
这项工作展示了用于神经元细胞培养的电和流体活性三维微电极阵列（3D MEA）的设计、制造、包装、表征和功能。该装置的成功功能意味着这个基本概念——用分层方法构建三维阵列——可以作为新的神经电极阵列家族的基础。3D MEA原型由一堆单独图案的薄膜组成，这些薄膜形成一个细胞室，有助于维持和记录大鼠皮层神经元长期三维网络的电活动。硅电极层包含一个用于神经分支、生长和网络形成的聚合物网格。沿着这些电极层的壁有暴露的金电极，可以记录和刺激神经元的电活动。有机硅弹性体微流控层提供了一种将游离神经元加载到结构中的方法，并作为营养供应和通气的人工血管。流体层还可以作为微电极的绝缘材料。细胞在3D MEA中存活长达28天，并在此期间进行了自发和诱发电记录。微流控能力通过药物河豚毒素的流动来影响培养物的活性。
This work demonstrates the design, fabrication, packaging, characterization, and functionality of an electrically and fluidically active three-dimensional micro-electrode array (3D MEA) for use with neuronal cell cultures. The successful function of the device implies that this basic concept—construction of a 3D array with a layered approach—can be utilized as the basis for a new family of neural electrode arrays. The 3D MEA prototype consists of a stack of individually patterned thin films that form a cell chamber conducive to maintaining and recording the electrical activity of a long-term three-dimensional network of rat cortical neurons. Silicon electrode layers contain a polymer grid for neural branching, growth, and network formation. Along the walls of these electrode layers lie exposed gold electrodes which permit recording and stimulation of the neuronal electrical activity. Silicone elastomer micro-fluidic layers provide a means for loading dissociated neurons into the structure and serve as the artificial vasculature for nutrient supply and aeration. The fluidic layers also serve as insulation for the micro-electrodes. Cells have been shown to survive in the 3D MEA for up to 28 days, with spontaneous and evoked electrical recordings performed in that time. The micro-fluidic capability was demonstrated by flowing in the drug tetrotodoxin to influence the activity of the culture.
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影响因子：
2.7
作者：
Jo, BH;Van Lerberghe, LM;Beebe, DJ
通讯作者：
Beebe, DJ
DOI：
10.1073/pnas.95.25.14821
发表时间：
1998-12-08
影响因子：
11.1
作者：
Wang, F;Weaver, VM;Bissell, MJ
通讯作者：
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
2.5
作者：
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通讯作者：
Fetz, Eberhard E.
影响因子：
4
作者：
Irons, Hillary R.;Cullen, D. Kacy;LaPlaca, Michelle C.
通讯作者：
LaPlaca, Michelle C.