Automated tracking of animal posture and movement during exploration and sensory orientation behaviors.
Automated tracking of animal posture and movement during exploration and sensory orientation behaviors.
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DOI：
10.1371/journal.pone.0041642
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Louis M
中科院分区：
文献类型：
--
作者：
Gomez-Marin A;Partoune N;Stephens GJ;Louis M
有机体的神经功能主要反映在它能够做出的行为上。以高分辨率和自动化方式定量测量行为提供了关于潜在神经电路计算的有价值的信息。因此，用于动物跟踪的计算机视觉应用正在成为系统神经科学中遗传、分子和电生理表征的关键补充工具包。我们提出了感觉定向软件(SOS)来测量行为和推断感觉体验的相关性。SOS是一个简单而通用的系统，用于跟踪二维环境中单个动物的身体姿势和运动。在感觉场景存在的情况下，跟踪动物的传感器的轨迹及其姿势的演变为研究感觉运动整合提供了一个定量的框架。为了说明SOS的实用性，我们研究了果蝇幼虫对气味、温度和光照梯度的定位行为。我们发现，SOS适合于对包括扁虫、鱼类和老鼠在内的各种生物进行高分辨率的行为跟踪。我们的工作有助于不断增加的行为分析工具，用于收集丰富和细粒度的数据，以得出和测试关于神经系统功能的假设。通过开放获取我们的代码并记录软件设计，我们的目标是鼓励广泛的非专家社区对SOS进行调整，以适应他们特定的模型生物体和感兴趣的问题。
The nervous functions of an organism are primarily reflected in the behavior it is capable of. Measuring behavior quantitatively, at high-resolution and in an automated fashion provides valuable information about the underlying neural circuit computation. Accordingly, computer-vision applications for animal tracking are becoming a key complementary toolkit to genetic, molecular and electrophysiological characterization in systems neuroscience. We present Sensory Orientation Software (SOS) to measure behavior and infer sensory experience correlates. SOS is a simple and versatile system to track body posture and motion of single animals in two-dimensional environments. In the presence of a sensory landscape, tracking the trajectory of the animal's sensors and its postural evolution provides a quantitative framework to study sensorimotor integration. To illustrate the utility of SOS, we examine the orientation behavior of fruit fly larvae in response to odor, temperature and light gradients. We show that SOS is suitable to carry out high-resolution behavioral tracking for a wide range of organisms including flatworms, fishes and mice. Our work contributes to the growing repertoire of behavioral analysis tools for collecting rich and fine-grained data to draw and test hypothesis about the functioning of the nervous system. By providing open-access to our code and documenting the software design, we aim to encourage the adaptation of SOS by a wide community of non-specialists to their particular model organism and questions of interest.
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影响因子：
48
作者：
Gershow, Marc;Berck, Matthew;Mathew, Dennis;Luo, Linjiao;Kane, Elizabeth A.;Carlson, John R.;Samuel, Aravinthan D. T.
通讯作者：
Samuel, Aravinthan D. T.
影响因子：
2.2
作者：
Crespo, Jose E.;Lazzari, Claudio R.;Castelo, Marcela K.
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Castelo, Marcela K.
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作者：
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影响因子：
5.3
作者：
Iino, Yuichi;Yoshida, Kazushi
通讯作者：
Yoshida, Kazushi
影响因子：
5.3
作者：
Gomez-Marin A;Duistermars BJ;Frye MA;Louis M
通讯作者：
Louis M