Histological studies of the effects of chronic implantation of ceramic-based microelectrode arrays and microdialysis probes in rat prefrontal cortex.
Histological studies of the effects of chronic implantation of ceramic-based microelectrode arrays and microdialysis probes in rat prefrontal cortex.
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DOI：
10.1016/j.brainres.2009.06.084
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发表时间：
2009-09-29
期刊：
影响因子：
2.9
通讯作者：
Gerhardt GA
中科院分区：
文献类型：
--
作者：
Hascup ER;af Bjerkén S;Hascup KN;Pomerleau F;Huettl P;Strömberg I;Gerhardt GA
神经递质测量装置的慢性植入对于持续多天的清醒行为研究至关重要。关于长期植入对周围脑实质的影响以及该组织功能特性的改变，知之甚少。我们研究了慢性植入陶瓷微电极阵列（MEA）或微透析探针所产生的组织损伤程度。使用神经元（甲酚紫）、星形胶质细胞（GFAP）、小胶质细胞（Iba-1）、神经元能神经纤维（VGLUT 1）和血脑屏障（SMI-71）的染色剂对固定的组织进行组织学研究。尼氏染色显示，与MEA相比，微透析植入物的组织体损失明显。MEA产生了从轨道延伸50-100 µm的轻度神经胶质增生，从第3天开始受影响区域发生显著变化。相比之下，微透析探针产生了从轨道延伸200-300 µm的神经胶质增生，这在第3天和第7天是显著的。小胶质细胞和神经胶质纤维的标记物支持MEA产生最小的损伤，仅在第3天和第7天发生显著变化，一个月后恢复到对照水平。SMI-71染色支持微透析探针和MEA在1周内的血脑屏障完整性。该数据支持陶瓷MEA小尺寸和生物相容性对于准确测量完整脑中的神经递质水平是必要的。MEA的最小侵入性减少了组织损失，允许对脑活动进行长期（>6个月）电化学和电生理学监测。
Chronic implantation of neurotransmitter measuring devices is essential for awake, behavioral studies occurring over multiple days. Little is known regarding the effects of long term implantation on surrounding brain parenchyma and the resulting alterations in the functional properties of this tissue. We examined the extent of tissue damage produced by chronic implantation of either ceramic microelectrode arrays (MEAs) or microdialysis probes. Histological studies were carried out on fixed tissues using stains for neurons (cresyl violet), astrocytes (GFAP), microglia (Iba-1), glutamatergic nerve fibers (VGLUT1), and the blood-brain barrier (SMI-71). Nissl staining showed pronounced tissue body loss with microdialysis implants compared to MEAs. The MEAs produced mild gliosis extending 50–100 µm from the tracks, with a significant change in the affected areas starting at 3 days. By contrast, the microdialysis probes produced gliosis extending 200–300 µm from the track, which was significant at 3 and 7 days. Markers for microglia and glutamatergic fibers supported that the MEAs produce minimal damage with significant changes occurring only at 3 and 7 days that return to control levels by one month. SMI-71 staining supported integrity of the blood brain barrier out to 1 week for both the microdialysis probes and the MEAs. This data support that the ceramic MEAs small size and biocompatibility are necessary to accurately measure neurotransmitter levels in the intact brain. The minimal invasiveness of the MEAs reduce tissue loss, allowing for long term (>6 month) electrochemical and electrophysiological monitoring of brain activity.
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影响因子：
3.4
作者：
Joyce, B. Matthew;Glaser, Paul E. A.;Gerhardt, Greg A.
通讯作者：
Gerhardt, Greg A.
DOI：
10.1006/bbrc.1996.1112
发表时间：
1996-07-25
影响因子：
3.1
作者：
Imai, Y;Ibata, I;Kohsaka, S
通讯作者：
Kohsaka, S
影响因子：
3
作者：
Borland, LM;Shi, GY;Michael, AC
通讯作者：
Michael, AC
影响因子：
3.7
作者：
BOURDELAIS, AJ;DEUTCH, AY
通讯作者：
DEUTCH, AY
影响因子：
12.6
作者：
Burmeister, JJ;Palmer, M;Gerhardt, GA
通讯作者：
Gerhardt, GA