Transforming growth factor-beta increases the expression of vascular smooth muscle cell markers in human multi-lineage progenitor cells.
Transforming growth factor-beta increases the expression of vascular smooth muscle cell markers in human multi-lineage progenitor cells.
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DOI：
10.12659/msm.881439
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发表时间：
2011-02-25
期刊：
影响因子：
--
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Yang H;Zhang L;Weakley SM;Lin PH;Yao Q;Chen C
血管平滑肌细胞（SMC）分化是血管修复和组织工程的重要组成部分。然而，目前用于研究SMC分化的细胞模型存在一些局限性。多谱系祖细胞（MLPCs）来源于人脐带血，由单个细胞克隆而来。本研究的目的是探讨在转化生长因子β1（TGF - β1）诱导下，MLPCs是否能在体外分化为SMCs。
MLPCs在含1%胎牛血清的间充质干细胞培养基中，分别在不添加和添加TGF - β1（1和5 ng/mL）的条件下处理7天。从MLPCs中提取总RNA，并进行半定量实时聚合酶链反应以检测以下mRNA水平：早期和晚期SMC特异性标志物、两种内皮细胞（EC）特异性标志物、内皮祖细胞（EPC）标志物CD34、TGF - β1辅助蛋白CD105以及黏附分子CD146。
TGF - β1（1 ng/mL）显著提高了SMC特异性标志物SM22α、钙调蛋白 - 1、SMα - 肌动蛋白、钙调素结合蛋白、原肌球蛋白和肌球蛋白轻链激酶以及黏附分子CD146的mRNA水平。在MLPCs经TGF - β1（1 ng/mL）处理后，内皮细胞特异性标志物血管内皮钙黏蛋白和血管内皮生长因子受体 - 2、内皮祖细胞标志物CD34以及TGF - β1辅助蛋白CD105的mRNA水平显著降低。5 ng/mL的TGF - β1对这些基因的表达有类似影响。
本研究表明，在TGF - β1存在的情况下，MLPCs发生SMC谱系分化，这表明MLPCs是一种有前景的用于SMC谱系分化研究的细胞模型，可能有助于血管修复和组织工程的进展。
Vascular smooth muscle cell (SMC) differentiation is an essential component of vascular repair and tissue engineering. However, currently used cell models for the study of SMC differentiation have several limitations. Multi-lineage progenitor cells (MLPCs) originate from human umbilical cord blood and are cloned from a single cell. The object of this study was to investigate whether MLPCs could differentiate into SMCs in vitro with induction by transforming growth factor β1 (TGF-β1). MLPCs were treated without or with TGF-β1 (1 and 5 ng/mL) in mesenchymal stem cell media plus 1% FBS for 7 days. Total RNA was isolated from the MLPCs, and semi-quantitative real-time PCR was performed to test the following mRNA levels: early and late phase SMC-specific markers, two endothelial cell (EC)-specific markers, endothelial progenitor cell (EPC) marker CD34, TGF-β1 accessory protein CD105, and adhesion molecule CD146. TGF-β1 (1 ng/mL) significantly increased the mRNA levels of SMC-specific markers SM22α, calponin-1, SM α-actin, caldesmon, tropomyosin and MLCK as well as adhesion molecule CD146. The mRNA levels of EC-specific markers VE-cadherin and VEGFR-2, EPC marker CD34 and TGF-β1 accessory protein CD105 were decreased significantly, after MLPC were treated with TGF-β1 (1 ng/mL). TGF-β1 at 5 ng/mL showed similar effect on the expression of these genes. This study demonstrates that in the presence of TGF-β1, MLPCs undergo SMC lineage differentiation indicating that MLPCs are a promising cell model for SMC lineage differentiation studies, which may contribute to advances in vascular repair and tissue engineering.
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影响因子：
64.8
作者：
WRANA, JL;ATTISANO, L;MASSAGUE, J
通讯作者：
MASSAGUE, J
影响因子：
3.3
作者：
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4
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影响因子：
37.8
作者：
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通讯作者：
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影响因子：
3.7
作者：
Caglayan E;Romeo GR;Kappert K;Odenthal M;Südkamp M;Body SC;Shernan SK;Hackbusch D;Vantler M;Kazlauskas A;Rosenkranz S
通讯作者：
Rosenkranz S