Iron facilitates the RetS-Gac-Rsm cascade to inversely regulate protease IV (piv) expression via the sigma factor PvdS in Pseudomonas aeruginosa.
Iron facilitates the RetS-Gac-Rsm cascade to inversely regulate protease IV (piv) expression via the sigma factor PvdS in Pseudomonas aeruginosa.
复制标题
铁促进 RetS-Gac-Rsm 级联反应，通过铜绿假单胞菌中的 sigma 因子 PvdS 反向调节蛋白酶 IV (piv) 的表达。
DOI：
10.1111/1462-2920.15270
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发表时间：
2020-10
影响因子：
5.1
通讯作者：
Liang Haihua
中科院分区：
文献类型：
--
作者：
Peng Juan;Chen Gukui;Xu Xuejie;Wang Tietao;Liang Haihua
铜绿假单胞菌产生几种蛋白酶，如弹性蛋白酶（LasB蛋白酶）、LasA蛋白酶和蛋白酶IV（PIV），它们被认为是感染期间的重要毒力因子。LasA和LasB表达的调节剂已经被鉴定和充分表征;然而，蛋白酶IV（PIV）的这种调节的分子细节在很大程度上仍然未知。在这里，我们描述了蛋白酶IV和RetS/Rsm信号通路之间的相互作用，这在控制多种毒力因子的产生以及从嗜热菌到生物膜生活方式的转变中起着核心作用。我们发现，与野生型亲本相比，在限制性条件下生长的ΔretS或ΔrsmA菌株中piv的表达降低，但在丰富条件下生长的ΔretS或ΔrsmA突变体中piv的表达被诱导。我们比较了piv在不同条件下的表达，发现铁促进RetS/Rsm系统导致这种逆调节。此外，我们发现，RetS/Rsm途径调节PIV生产依赖于替代西格玛因子PvdS。总的来说，这项研究扩展了对RetS/Rsm调节级联响应环境信号的理解，并提供了铜绿假单胞菌如何适应复杂条件的见解。本文受版权保护。All rights reserved.
Pseudomonas aeruginosa produces several proteases, such as an elastase (LasB protease), a LasA protease, and protease IV (PIV), which are thought as significant virulence factors during infection. Regulators of LasA and LasB expression have been identified and well characterized; however, the molecular details of this regulation of protease IV (PIV) remained largely unknown. Here, we describe the interaction between protease IV and the RetS/Rsm signaling pathway, which plays a central role in controlling the production of multiple virulence factors as well as the switch from planktonic to biofilm lifestyle. We show that the expression of piv was reduced in ΔretS or ΔrsmA strain grown under restrictive conditions but was induced in ΔretS or ΔrsmA mutant grown under rich conditions as compared to wild-type parent. We compare the expression of piv under various conditions and found that iron facilitates RetS/Rsm system to lead this inverse regulation. Moreover, we reveal that the RetS/Rsm pathway regulates PIV production dependent on the alternative sigma factor PvdS. Collectively, this study extends the understanding of the RetS/Rsm regulatory cascade in response to environmental signals and provides insights into how P. aeruginosa adapts to the complex conditions. This article is protected by copyright. All rights reserved.
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