Locus number estimation of MHC class II B in stone flounder and Japanese flounder.
Locus number estimation of MHC class II B in stone flounder and Japanese flounder.
复制标题
石鲽和牙鲆中 MHC II B 类基因座数估计
DOI：
10.3390/ijms16036000
复制
发表时间：
2015-03-13
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Jiang J;Li C;Zhang Q;Wang X
主要组织相容性复合体 (MHC) 家族的成员在免疫系统中很重要。为了揭示它们复杂的基因结构，人们付出了巨大的努力。但许多现有研究都集中在 MHC 基因的部分序列上。在本研究中，通过基因克隆和测序，我们鉴定了两种比目鱼石鲽（Kareius bicoloratus）和纯合二倍体日本牙鲆（Paralichthys olivaceus）的 cDNA 序列和 MHC II B 类 DNA 序列。从8个石鲽个体中获得11个cDNA序列，大部分多态性位点分布在外显子2和3上。从这8个个体的DNA片段中鉴定出28个等位基因。从贝叶斯推理系统发育树可以推断，石鲽体内至少存在4个MHC II B类基因座。对一个个体的详细全长 DNA 序列进行了分析，揭示了不同基因座内含子长度的差异。从一个纯合二倍体日本牙鲆个体中鉴定出四种不同的 cDNA 序列，这意味着至少存在四个基因座。 cDNA序列与DNA序列的比较证实，日本牙鲆的该基因存在6个外显子，这是鲽形目鱼类共有的特征。我们的结果证明了MHC II B类的多位点特征。我们获得的序列将为进一步的研究提供详细、系统的数据。
Members of major histocompatibility complex (MHC) family are important in immune systems. Great efforts have been made to reveal their complicated gene structures. But many existing studies focus on partial sequences of MHC genes. In this study, by gene cloning and sequencing, we identified cDNA sequences and DNA sequences of the MHC class II B in two flatfishes, stone flounder (Kareius bicoloratus) and homozygous diploid Japanese flounder (Paralichthys olivaceus). Eleven cDNA sequences were acquired from eight stone flounder individuals, and most of the polymorphic sites distributed in exons 2 and 3. Twenty-eight alleles were identified from the DNA fragments in these eight individuals. It could be deduced from their Bayesian inference phylogenetic tree that at least four loci of MHC class II B exist in stone flounder. The detailed whole-length DNA sequences in one individual were analyzed, revealing that the intron length varied among different loci. Four different cDNA sequences were identified from one homozygous diploid Japanese flounder individual, implying the existence of at least four loci. Comparison of the cDNA sequences to the DNA sequence confirmed that six exons existed in this gene of Japanese flounder, which was a common feature shared by Pleuronectiformes fishes. Our results proved the multi-locus feature of MHC class II B. The sequences we obtained would provide detailed and systematic data for further research.
登录
查看更多内容
DOI：
10.1098/rspb.1995.0048
发表时间：
1995-03-22
影响因子：
4.7
作者：
FIGUEROA, F;ONO, H;KLEIN, J
通讯作者：
KLEIN, J
影响因子：
3.2
作者：
CrouauRoy, B;Bouzekri, N;CambonThomsen, A
通讯作者：
CambonThomsen, A
影响因子：
2.4
作者：
Dong, Zhong-Dian;Zhao, Yan;Wang, Hui
通讯作者：
Wang, Hui
影响因子：
2.2
作者：
Li, C.;Yu, Y.;Zhang, Q.
通讯作者：
Zhang, Q.
两个主要组织相容性 (MH) II A 类基因的鉴定及其与半滑舌鳎 (Cynoglossus semilaevis) 弧菌感染的关联
影响因子：
1.6
作者：
Li Chunmei;Wang Xubo;Yu Haiyang
通讯作者：
Yu Haiyang