Increased expression of the MALE STERILITY1 transcription factor gene results in temperature-sensitive male sterility in barley.
Increased expression of the MALE STERILITY1 transcription factor gene results in temperature-sensitive male sterility in barley.
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DOI：
10.1093/jxb/eraa382
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发表时间：
2020-10-22
影响因子：
6.9
通讯作者：
Wilson ZA
中科院分区：
文献类型：
--
作者：
Fernández-Gómez J;Talle B;Wilson ZA
HvMS 1在大麦中的过表达导致温敏雄性不育，这是由于持续的绒毡层、花药开裂的失败和粘性花粉;然而，在较高温度下的生长挽救了育性。了解肥力控制对作物产量和育种至关重要;这对于杂交育种利用由此产生的杂交优势尤为重要。已经采用了不同的杂交育种系统;然而，这些都是具有挑战性的和特定的作物。具有环境可逆育性的突变体为杂交育种提供了宝贵的机会。大麦HvMS 1基因编码一个PHD-指转录因子，在花药绒毡层中表达，这是花粉发育所必需的，当在大麦中过表达时会导致完全雄性不育。这种雄性不育至少部分是由于不完全的绒毡层退化导致的花药不开裂，花药无法打开，以及正常生长条件下（15 °C）的粘性花粉。然而，当植物在>20 °C的温度下生长时，或者当在花粉有丝分裂I之前的开花期间转移到>20 °C时，开裂和育性恢复，在后期阶段的转移不能挽救体内的育性。据我们所知，这是大麦温敏雄性不育的首次报道。这为了解温度对花粉发育的影响以及环境可切换杂交育种系统的潜在应用提供了机会;它还提供了一种不需要去雄以促进回交的“雌性”雄性不育育种工具。
Overexpression of HvMS1 in barley results in thermosensitive male sterility, due to persistent tapetum, failure of anther dehiscence, and sticky pollen; however, fertility is rescued by growth at higher temperatures. Understanding the control of fertility is critical for crop yield and breeding; this is particularly important for hybrid breeding to capitalize upon the resultant hybrid vigour. Different hybrid breeding systems have been adopted; however, these are challenging and crop specific. Mutants with environmentally reversible fertility offer valuable opportunities for hybrid breeding. The barley HvMS1 gene encodes a PHD-finger transcription factor that is expressed in the anther tapetum, which is essential for pollen development and causes complete male sterility when overexpressed in barley. This male sterility is due at least in part to indehiscent anthers resulting from incomplete tapetum degeneration, failure of anther opening, and sticky pollen under normal growth conditions (15 °C). However, dehiscence and fertility are restored when plants are grown at temperatures >20 °C, or when transferred to >20 °C during flowering prior to pollen mitosis I, with transfer at later stages unable to rescue fertility in vivo. As far as we are aware, this is the first report of thermosensitive male sterility in barley. This offers opportunities to understand the impact of temperature on pollen development and potential applications for environmentally switchable hybrid breeding systems; it also provides a ‘female’ male-sterile breeding tool that does not need emasculation to facilitate backcrossing.
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影响因子：
6.9
作者：
Gómez JF;Wilson ZA
通讯作者：
Wilson ZA
DOI：
10.1111/j.1469-8137.2012.04329.x
发表时间：
2012-12
期刊：
The New phytologist
影响因子：
--
作者：
Nelson MR;Band LR;Dyson RJ;Lessinnes T;Wells DM;Yang C;Everitt NM;Jensen OE;Wilson ZA
通讯作者：
Wilson ZA
影响因子：
9.4
作者：
KEIJZER, CJ
通讯作者：
KEIJZER, CJ
DOI：
10.1073/pnas.0909097107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Nair, Sudha K.;Wang, Ning;Komatsuda, Takao
通讯作者：
Komatsuda, Takao
影响因子：
1.9
作者：
Adugna, A;Nanda, GS;Bains, NS
通讯作者：
Bains, NS