Single-cell RNA sequencing of adult rat testes after Leydig cell elimination and restoration.
Single-cell RNA sequencing of adult rat testes after Leydig cell elimination and restoration.
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Leydig 细胞消除和恢复后成年大鼠睾丸的单细胞 RNA 测序
DOI：
10.1038/s41597-022-01225-5
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发表时间：
2022-03-25
期刊：
影响因子：
9.8
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Guan X;Ji M;Wen X;Huang F;Zhao X;Chen D;Shao J;Wang J;Xie J;Tian J;Lin H;Duan P;Zirkin BR;Su Z;Chen H
精子发生是一个高效、复杂和高度组织化的增殖和分化过程，它依赖于多种因素，包括间质细胞产生的睾酮。尽管睾酮在精子发生中所起的关键作用已经得到了很好的认识，但其作用机制仍不完全清楚，部分原因是无法特异性和准确地监测发育中的生殖细胞内对睾酮的依赖变化。在这里，我们提供了10,983只成年大鼠睾丸细胞的单细胞RNA测序数据，这些细胞在用乙二甲烷磺酸盐处理后，暂时消除了间质细胞。间质细胞的消除和恢复代表了一个完整的睾酮耗竭和恢复周期。该数据集包括从精原细胞到精子的所有发育中的生殖细胞，应该被证明对描述发育中的生殖细胞、它们的调控网络和新的细胞特异性标记是有用的。该数据集对于探索雄激素环境对精子发生调控的影响尤其有用。由于这是第一个针对大鼠睾丸的单细胞RNA-Seq数据集，因此也可以作为未来研究的参考。
Spermatogenesis is an efficient, complex, and highly organized proliferation and differentiation process that relies on multiple factors including testosterone produced by the Leydig cells. Although the critical role played by testosterone in spermatogenesis is well recognized, the mechanism by which it works is still not completely understood, partially due to the inability to specifically and precisely monitor testosterone-dependent changes within developing germ cells. Here we present single-cell RNA sequencing data from10,983 adult rat testicular cells after the rats were treated with ethanedimethanesulfonate, which temporarily eliminates Leydig cells. The elimination and recovery of Leydig cells represented a complete testosterone depletion and restoration cycle. The dataset, which includes all developing germ cells from spermatogonia to spermatozoa, should prove useful for characterizing developing germ cells, their regulatory networks, and novel cell-specific markers. The dataset should be particularly useful for exploring the effects of the androgen environment on the regulation of spermatogenesis. As this is the first single-cell RNA-Seq dataset for rat testes, it can also serve as a reference for future studies.
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DOI：
10.1098/rstb.2010.0026
发表时间：
2010-05-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Phillips BT;Gassei K;Orwig KE
通讯作者：
Orwig KE
影响因子：
5.8
作者：
Prokai D;Pudasaini A;Kanchwala M;Moehlman AT;Waits AE;Chapman KM;Chaudhary J;Acevedo J;Keller P;Chao X;Carr BR;Hamra FK
通讯作者：
Hamra FK
影响因子：
11.8
作者：
Green CD;Ma Q;Manske GL;Shami AN;Zheng X;Marini S;Moritz L;Sultan C;Gurczynski SJ;Moore BB;Tallquist MD;Li JZ;Hammoud SS
通讯作者：
Hammoud SS
DOI：
10.1073/pnas.0709854105
发表时间：
2008-06-17
影响因子：
11.1
作者：
Johnston, Daniel S.;Wright, William W.;Jelinsky, Scott A.
通讯作者：
Jelinsky, Scott A.
影响因子：
4.6
作者：
Holdcraft, RW;Braun, RE
通讯作者：
Braun, RE