Cellular gibberellin dynamics govern indeterminate nodule development, morphology and function
Cellular gibberellin dynamics govern indeterminate nodule development, morphology and function
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细胞赤霉素动力学控制不确定的结节发育、形态和功能
DOI：
10.1101/2023.09.09.556959
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Alexander M. Jones
中科院分区：
文献类型：
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作者：
Colleen Drapek;Nadiatul Radzman;Annalisa Rizza;K. Schiessl;Fabio Dos Santos Barbosa;Jiangqi Wen;G. Oldroyd;Alexander M. Jones
在营养缺乏期间，植物可以调整其发展策略，以最大限度地提高生存机会。这种可塑性的发展是由激素调节，介导的环境线索和发育输出之间的关系。在豆科植物中，与固氮细菌（根瘤菌）的内共生是以铵的形式为植物提供氮的关键适应。根瘤菌被安置在侧根衍生的称为根瘤的器官中，这些根瘤维持有利于这些细菌中的固氮酶的环境。几种植物激素对调节根瘤的形成很重要，赤霉素（GA）的作用既有积极的，也有消极的。在这项研究中，我们确定了细胞的位置和功能的生物活性GA在结节器官形成过程中使用遗传编码的第二代GA生物传感器，GPS2。我们发现，内源性生物活性GA积累在当地的网站结节原基，显着增加皮层细胞层，坚持通过细胞分裂和维持积累在成熟的结节分生组织。我们表明，通过错误表达的GA分解代谢酶，抑制GA积累，GA作为一个积极的调节结节的生长和发育。此外，通过干扰生物合成基因表达来增加或减少GA可以分别增加或减少结节的大小。这是独特的侧根形成，一个发展计划，共享共同的器官发生调节。我们链接GA到一个更广泛的基因调控程序显示，细胞分裂素以及结节身份基因诱导和维持GA积累结节功能所必需的。
During nutrient scarcity, plants can adapt their developmental strategy to maximize their chance of survival. Such plasticity in development is underpinned by hormonal regulation, which mediates the relationship between environmental cues and developmental outputs. In legumes, endosymbiosis with nitrogen fixing bacteria (rhizobia) is a key adaptation for supplying the plant with nitrogen in the form of ammonium. Rhizobia are housed in lateral root-derived organs termed nodules that maintain an environment conducive to Nitrogenase in these bacteria. Several phytohormones are important for regulating the formation of nodules, with both positive and negative roles proposed for gibberellin (GA). In this study, we determined the cellular location and function of bioactive GA during nodule organogenesis using a genetically-encoded second generation GA biosensor, GPS2. We found endogenous bioactive GA accumulates locally at the site of nodule primordia, increasing dramatically in the cortical cell layers, persisting through cell divisions and maintaining accumulation in the mature nodule meristem. We show, through mis-expression of GA catabolic enzymes that suppress GA accumulation, that GA acts as a positive regulator of nodule growth and development. Furthermore, increasing or decreasing GA through perturbation of biosynthesis gene expression can increase or decrease the size of nodules, respectively. This is unique from lateral root formation, a developmental program that shares common organogenesis regulators. We link GA to a wider gene regulatory program by showing that cytokinin as well as nodule-identity genes induce and sustain GA accumulation necessary for nodule function.
DOI：
10.1101/2023.02.12.528179
发表时间：
2023-02
期刊：
bioRxiv
影响因子：
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作者：
K. Schiessl;T. Lee;M. Orvošová;M. Bueno-Batista;N. Stuer;P. Bailey;K. Mysore;J. Wen;G. Oldroyd
通讯作者：
K. Schiessl;T. Lee;M. Orvošová;M. Bueno-Batista;N. Stuer;P. Bailey;K. Mysore;J. Wen;G. Oldroyd
为了可持续的粮食安全，我们能否换一种方式从化学固氮转向生物固氮？
影响因子：
9.8
作者：
通讯作者：
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