Thermal adaptation of cellular membranes in natural populations of Drosophila melanogaster.
Thermal adaptation of cellular membranes in natural populations of Drosophila melanogaster.
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DOI：
10.1111/1365-2435.12264
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发表时间：
2014-08-01
影响因子：
5.2
通讯作者：
Montooth KL
中科院分区：
文献类型：
--
作者：
Cooper BS;Hammad LA;Montooth KL
温度的变化会破坏细胞膜的流动性，这会对膜的完整性和细胞过程产生负面影响。许多外温动物，包括黑腹果蝇（Meigen），调整其膜的甘油磷脂组成，以恢复最佳的流动性，当温度变化时，一种被称为同源粘性适应的性状可塑性。现有的数据表明，可塑性的甘油磷脂磷脂酰乙醇胺（PE）和磷脂酰胆碱（PC）的相对丰度的基础细胞适应的时间变化的热环境。例如，D.与恒温下进化的种群相比，在随时间变化的温度下进化的黑腹鱼具有更大的PE与PC之比（PE/PC）的发育可塑性和更大的繁殖力。在这里，我们将这项工作扩展到D.通过评估从美国佛蒙特州、印第安纳州和北卡罗来纳州分离的基因型中不同生命阶段甘油磷脂组成的热可塑性，对黑腹果蝇进行了研究。我们还量化了发育和成人（可逆）可塑性之间的协方差，以及成人之间的膜对冷热变化的反应。正如所预测的生理模型的homeov粘适应，苍蝇从所有人口减少PE/PC和程度的脂质不饱和反应温暖的温度。此外，这些人群的膜可塑性程度不同。苍蝇从最多变的热环境（佛蒙特州，美国）减少PE/PC在更大程度上比其他人群在温暖的温度下开发时，一种模式，符合我们以前的观察实验室进化的人群。我们还发现，PE/PC的发育可塑性和成人可塑性在基因型之间存在协变，但成人对冷热温度变化的反应不存在协变。结合我们以前对实验室进化种群的观察，我们的研究结果表明PE/PC的发育可塑性是随时间变化的环境中热适应的一种机制。虽然我们对塑料对温度反应的遗传基础知之甚少，但我们的观察表明，环境敏感和环境特异性等位基因都有助于膜的热适应，并且当成年环境与发育过程中经历的环境不同时，可塑性的成本可能会增加。
Changes in temperature disrupt the fluidity of cellular membranes, which can negatively impact membrane integrity and cellular processes. Many ectotherms, including Drosophila melanogaster (Meigen), adjust the glycerophospholipid composition of their membranes to restore optimal fluidity when temperatures change, a type of trait plasticity termed homeoviscous adaptation. Existing data suggest that plasticity in the relative abundances of the glycerophospholipids phosphatidylethanolamine (PE) and phosphatidylcholine (PC) underlies cellular adaptation to temporal variability in the thermal environment. For example, laboratory populations of D. melanogaster evolved in the presence of temporally variable temperatures have greater developmental plasticity of the ratio of PE to PC (PE/PC) and greater fecundity than do populations evolved at constant temperatures. Here, we extend this work to natural populations of D. melanogaster by evaluating thermal plasticity of glycerophospholipid composition at different life stages, in genotypes isolated from Vermont, Indiana and North Carolina, USA. We also quantify the covariance between developmental and adult (reversible) plasticity, and between adult responses of the membrane to cool and warm thermal shifts. As predicted by physiological models of homeoviscous adaptation, flies from all populations decrease PE/PC and the degree of lipid unsaturation in response to warm temperatures. Furthermore, these populations have diverged in their degree of membrane plasticity. Flies from the most variable thermal environment (Vermont, USA) decrease PE/PC to a greater extent than do other populations when developed at a warm temperature, a pattern that matches our previous observation in laboratory-evolved populations. We also find that developmental plasticity and adult plasticity of PE/PC covary across genotypes, but that adult responses to cool and warm thermal shifts do not. When combined with our previous observations of laboratory-evolved populations, our findings implicate developmental plasticity of PE/PC as a mechanism of thermal adaptation in temporally variable environments. While little is known about the genetic bases of plastic responses to temperature, our observations suggest that both environmentally sensitive and environmentally specific alleles contribute to thermal adaptation of membranes, and that costs of plasticity may arise when the adult environment differs from that experienced during development.
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影响因子：
56.9
作者：
Dobrosotskaya, IY;Seegmiller, AC;Rawson, RB
通讯作者：
Rawson, RB
DOI：
10.1007/s003600050165
发表时间：
1998-08-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
影响因子：
--
作者：
Kostal, V;Simek, P
通讯作者：
Simek, P
影响因子：
3.8
作者：
MILLER, RR;YATES, JW;GEER, BW
通讯作者：
GEER, BW
影响因子：
5.2
作者：
Feder, ME;Blair, N;Figueras, H
通讯作者：
Figueras, H
影响因子：
3.3
作者：
GOMULKIEWICZ, R;KIRKPATRICK, M
通讯作者：
KIRKPATRICK, M