Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells.
Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells.
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细胞特异性转录组分析表明，成体支柱细胞和 Deiters 细胞表达耳蜗毛细胞特化的基因编码机制
DOI：
10.3389/fnmol.2018.00356
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发表时间：
2018
影响因子：
4.8
通讯作者：
He DZZ
中科院分区：
文献类型：
--
作者：
Liu H;Chen L;Giffen KP;Stringham ST;Li Y;Judge PD;Beisel KW;He DZZ
哺乳动物的听觉感觉上皮（Corti器）由毛细胞和支持细胞组成。毛细胞在顶膜、基底膜和突触膜中具有特化。这些特化介导机械传导、电和机械活动以及突触传递。支持细胞维持耳蜗的离子和化学环境的稳态，并有助于耳蜗分区的硬度。虽然支持细胞的自发增殖和转分化是再生反应的来源，以取代低级脊椎动物中丢失的毛细胞，但成年哺乳动物中的支持细胞不再保留这种能力。揭示支持细胞的基本生物学特性的重要的第一步是表征其细胞类型特异性转录组。使用RNA-seq，我们检查了1，000个柱细胞和1，000个Deiters细胞以及两种类型的毛细胞的转录组，这些细胞使用吸管技术从成年CBA/J小鼠耳蜗中单独收集。我们的目标是确定柱细胞和Deiters细胞（毛细胞替代的常见靶细胞）是否表达已知编码顶膜、基底膜和突触膜中毛细胞特化机制的基因。我们发现柱细胞和Deiters细胞都表达这些基因，其中柱细胞比Deiters细胞更类似于毛细胞。成体柱细胞和Deiters细胞表达与毛细胞特化同源的基因的事实为在毛细胞由于损伤而丢失后靶向这些细胞用于哺乳动物毛细胞替代提供了强有力的分子基础。
The mammalian auditory sensory epithelium, the organ of Corti, is composed of hair cells and supporting cells. Hair cells contain specializations in the apical, basolateral and synaptic membranes. These specializations mediate mechanotransduction, electrical and mechanical activities and synaptic transmission. Supporting cells maintain homeostasis of the ionic and chemical environment of the cochlea and contribute to the stiffness of the cochlear partition. While spontaneous proliferation and transdifferentiation of supporting cells are the source of the regenerative response to replace lost hair cells in lower vertebrates, supporting cells in adult mammals no longer retain that capability. An important first step to revealing the basic biological properties of supporting cells is to characterize their cell-type specific transcriptomes. Using RNA-seq, we examined the transcriptomes of 1,000 pillar and 1,000 Deiters' cells, as well as the two types of hair cells, individually collected from adult CBA/J mouse cochleae using a suction pipette technique. Our goal was to determine whether pillar and Deiters' cells, the commonly targeted cells for hair cell replacement, express the genes known for encoding machinery for hair cell specializations in the apical, basolateral, and synaptic membranes. We showed that both pillar and Deiters' cells express these genes, with pillar cells being more similar to hair cells than Deiters' cells. The fact that adult pillar and Deiters' cells express the genes cognate to hair cell specializations provides a strong molecular basis for targeting these cells for mammalian hair cell replacement after hair cells are lost due to damage.
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影响因子：
16.6
作者：
Burns JC;Kelly MC;Hoa M;Morell RJ;Kelley MW
通讯作者：
Kelley MW
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
2.8
作者：
Burgess, BJ;Adams, JC;Nadol, JB
通讯作者：
Nadol, JB
影响因子：
15.9
作者：
Kawashima, Yoshiyuki;Geleoc, Gwenaelle S. G.;Griffith, Andrew J.
通讯作者：
Griffith, Andrew J.
影响因子：
3.4
作者：
Furness, DN;Lehre, KP
通讯作者：
Lehre, KP