Environmental tobacco smoke effects on lung surfactant film organization.
Environmental tobacco smoke effects on lung surfactant film organization.
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DOI：
10.1016/j.bbamem.2008.11.021
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发表时间：
2009-02
影响因子：
3.4
通讯作者：
Pinkerton, Kent E.
中科院分区：
文献类型：
--
作者：
Stenger, Patrick C.;Alonso, Coralie;Zasadzinski, Joseph A.;Waring, Alan J.;Jung, Chun-Ling;Pinkerton, Kent E.
临床肺表面活性剂（LS）Curosurf或Survanta从水悬浮液吸附到空气-水界面的过程是从多双层聚集体通过多层膜到多层和单层域共存。暴露于环境烟草烟雾（ETS）改变了这一进程所示的朗缪尔等温线，荧光显微镜和原子力显微镜（AFM）。LS暴露于ETS 12小时后，Langmuir-Blodgett沉积膜的AFM图像显示，ETS减少了界面附近的材料量，并改变了表面活性剂在压缩过程中从界面去除的方式。对于固尔苏，ETS可防止循环过程中薄膜成分的细化;这会导致更高的最小表面张力。ETS还通过将凝聚相域的尺寸从8-12 μm减小到~ 2μm来改变固速苏膜的形态，表明域之间的线张力降低。Survanta薄膜的最小表面张力和形态受ETS暴露的影响较小，尽管与薄膜相关的材料量以与Curosurf相似的方式减少。荧光和质谱Survanta分散体含有天然牛SP-B与ETS处理表明蛋白质芳香族氨基酸残基侧链的氧化降解。从暴露于ETS的Survanta中分离的天然牛SP-C的分子量变化与脂蛋白的脱酰化一致。傅立叶变换红外光谱（FTIR）表征的疏水蛋白从ETS处理的Survanta分散体显示显着的变化与改变的表面活性和形态的脂质蛋白膜的SP-B和SP-C的构象。
Adsorption of the clinical lung surfactants (LS) Curosurf or Survanta from aqueous suspension to the air-water interface progresses from multi-bilayer aggregates through multilayer films to a coexistence between multilayer and monolayer domains. Exposure to environmental tobacco smoke (ETS) alters this progression as shown by Langmuir isotherms, fluorescence microscopy and atomic force microscopy (AFM). After 12 hours of LS exposure to ETS, AFM images of Langmuir-Blodgett deposited films show that ETS reduces the amount of material near the interface and alters how surfactant is removed from the interface during compression. For Curosurf, ETS prevents refining of the film composition during cycling; this leads to higher minimum surface tensions. ETS also changes the morphology of the Curosurf film by reducing the size of condensed phase domains from 8–12 μm to ~ 2μm, suggesting a decrease in the line tension between the domains. The minimum surface tension and morphology of the Survanta film are less impacted by ETS exposure, although the amount of material associated with the film is reduced in a similar way to Curosurf. Fluorescence and mass spectra of Survanta dispersions containing native bovine SP-B treated with ETS indicate the oxidative degradation of protein aromatic amino acid residue side chains. Native bovine SP-C isolated from ETS exposed Survanta had changes in molecular mass consistent with deacylation of the lipoprotein. Fourier Transform Infrared Spectroscopy (FTIR) characterization of the hydrophobic proteins from ETS treated Survanta dispersions show significant changes in the conformation of SP-B and SP-C that correlate with the altered surface activity and morphology of the lipid-protein film.
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DOI：
10.1164/ajrccm.163.2.2006009
发表时间：
2001-02-01
影响因子：
24.7
作者：
Gilliland, FD;Li, YF;Peters, JM
通讯作者：
Peters, JM
影响因子：
3.9
作者：
Alonso, C;Bringezu, F;Zasadzinski, JA
通讯作者：
Zasadzinski, JA
影响因子：
8.6
作者：
Ding, JQ;Warriner, HE;Zasadzinski, JA
通讯作者：
Zasadzinski, JA
影响因子：
3.4
作者：
Alonso, C;Waring, A;Zasadzinski, JA
通讯作者：
Zasadzinski, JA
影响因子：
3.4
作者：
Cruz, A;Vázquez, L;Pérez-Gil, J
通讯作者：
Pérez-Gil, J