Identification of Single Nucleotide Polymorphism in TaSBEIII and Development of KASP Marker Associated With Grain Weight in Wheat.
Identification of Single Nucleotide Polymorphism in TaSBEIII and Development of KASP Marker Associated With Grain Weight in Wheat.
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小麦TaSBEIII单核苷酸多态性的鉴定及与粒重相关的KASP标记的开发
DOI：
10.3389/fgene.2021.697294
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发表时间：
2021
影响因子：
3.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Irshad A;Guo H;Ur Rehman S;Wang X;Gu J;Xiong H;Xie Y;Zhao L;Zhao S;Wang C;Liu L
操纵淀粉合成相关基因对小麦籽粒重和产量有显著影响。淀粉分支酶（SBE）通过切割聚葡聚糖中的α-1,4链，并通过α-1,6链重新连接，从而催化分支点的形成。高等植物中存在三种SBE亚型（SBEI、SBEII和SBEIII），且SBE亚型的数量具有物种特异性。本研究扩增了小麦TaSBEIII基因的编码序列。对20份小麦品种的TaSBEIII基因组进行多序列比对后，在TaSBEIII- a中发现1个SNP，形成等位基因标记等位基因- t。以294 bp (C/T) SNP为基础，建立了区分千粒重（TGW）等位基因变异的KASP分子标记。使用来自中国的262份、巴基斯坦的153份、CIMMYT的53份以及17份二倍体和18份四倍体基因型对结果进行了验证。TaSBEIII-A等位基因变异与农艺性状的关联分析发现，TaSBEIII-A与中国迷你核心种质（MCC）的TGW相关。等位基因t的植株TGW高于等位基因c；因此，等位基因t是一个有利的等位基因变异。通过对MCC有利等位基因变异等位基因- t的频率分析，从1950年前（25%）增加到1960年代（45%），从1960年到1990年（80%）持续增加。结果表明，KASP标记可用于粒重改良，最终通过标记辅助选择在小麦育种中提高小麦产量。等位基因t的有利变异对提高源库并提产量具有重要意义。此外，新开发的KASP标记在不同遗传背景下得到验证，可以整合到高TGW小麦的育种试剂盒中。
Manipulation of genes involved in starch synthesis could significantly affect wheat grain weight and yield. The starch-branching enzyme (SBE) catalyzes the formation of branch points by cleaving the α-1,4 linkage in polyglucans and reattaching the chain via an α-1,6 linkage. Three types of SBE isoforms (SBEI, SBEII, and SBEIII) exist in higher plants, with the number of SBE isoforms being species-specific. In this study, the coding sequence of the wheat TaSBEIII gene was amplified. After the multiple sequence alignment of TaSBEIII genome from 20 accessions in a wheat diversity panel, one SNP was observed in TaSBEIII-A, which formed the allelic marker allele-T. Based on this SNP at 294 bp (C/T), a KASP molecular marker was developed to distinguish allelic variation among the wheat genotypes for thousand grain weight (TGW). The results were validated using 262 accessions of mini core collection (MCC) from China, 153 from Pakistan, 53 from CIMMYT, and 17 diploid and 18 tetraploid genotypes. Association analysis between TaSBEIII-A allelic variation and agronomic traits found that TaSBEIII-A was associated with TGW in mini core collection of China (MCC). The accessions possessing Allele-T had higher TGW than those possessing Allele-C; thus, Allele-T was a favorable allelic variation. By analyzing the frequency of the favorable allelic variation Allele-T in MCC, it increased from pre-1950 (25%) to the 1960s (45%) and increased continuously from 1960 to 1990 (80%). The results suggested that the KASP markers can be utilized in grain weight improvement, which ultimately improves wheat yield by marker-assisted selection in wheat breeding. The favorable allelic variation allele-T should be valuable in enhancing grain yield by improving the source and sink simultaneously. Furthermore, the newly developed KASP marker validated in different genetic backgrounds could be integrated into a breeding kit for screening high TGW wheat.
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