Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis.
Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis.
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DOI：
10.1186/1471-2180-4-16
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发表时间：
2004-04-21
期刊：
影响因子：
4.2
通讯作者：
Vaneechoutte M
中科院分区：
文献类型：
--
作者：
Verhelst R;Verstraelen H;Claeys G;Verschraegen G;Delanghe J;Van Simaey L;De Ganck C;Temmerman M;Vaneechoutte M
细菌性阴道病的发病机制在很大程度上仍然是难以捉摸的，虽然一些微生物，包括加德纳菌，被怀疑在这种疾病的病因学中发挥作用。最近，微生物生态系统的非培养分析已经证明了其在表征细菌种群多样性方面的有效性。在这里，我们报告了通过结合培养和基于PCR的方法来表征正常和受干扰的阴道微生物群所获得的结果。根据直接涂片的革兰氏染色，将来自健康女性（115例妊娠和35例非妊娠）的共150份阴道拭子样本分为I级（n = 112）、II级（n = 26）、III级（n = 9）或IV级（n = 3）。通过培养和克隆直接扩增后获得的16 S rRNA基因，研究了8份阴道拭子（3份I级，2份II级和3份III级）的阴道微生物群落组成，所有拭子均来自非妊娠女性。通过tDNA-PCR鉴定了46个培养分离株，分析了854个克隆的16 S rRNA基因片段，其中156个通过测序，总共产生38个种，包括9个推测为新的种，至少有5个种以前没有从阴道样品中分离。有趣的是，克隆显示，在五个非I级标本中的四个中，阴道奇异菌是丰富的。最后，应用种特异性PCR技术对A.阴道加德纳氏菌和阴道加德纳氏菌表明细菌性阴道病样品中两种菌的统计学显著共同出现。虽然历史上关于细菌性阴道病的文献主要集中在G。特别是迷走神经，这项研究的几个发现-如A。阴道菌群失调和几个新物种的存在表明，阴道菌群的组成及其与BV病因学的关系还有很多要了解。
The pathogenesis of bacterial vaginosis remains largely elusive, although some microorganisms, including Gardnerella vaginalis, are suspected of playing a role in the etiology of this disorder. Recently culture-independent analysis of microbial ecosystems has proven its efficacy in characterizing the diversity of bacterial populations. Here, we report on the results obtained by combining culture and PCR-based methods to characterize the normal and disturbed vaginal microflora. A total of 150 vaginal swab samples from healthy women (115 pregnant and 35 non-pregnant) were categorized on the basis of Gram stain of direct smear as grade I (n = 112), grade II (n = 26), grade III (n = 9) or grade IV (n = 3). The composition of the vaginal microbial community of eight of these vaginal swabs (three grade I, two grade II and three grade III), all from non-pregnant women, were studied by culture and by cloning of the 16S rRNA genes obtained after direct amplification. Forty-six cultured isolates were identified by tDNA-PCR, 854 cloned 16S rRNA gene fragments were analysed of which 156 by sequencing, yielding a total of 38 species, including 9 presumptively novel species with at least five species that have not been isolated previously from vaginal samples. Interestingly, cloning revealed that Atopobium vaginae was abundant in four out of the five non-grade I specimens. Finally, species specific PCR for A. vaginae and Gardnerella vaginalis pointed to a statistically significant co-occurrence of both species in the bacterial vaginosis samples. Although historically the literature regarding bacterial vaginosis has largely focused on G. vaginalis in particular, several findings of this study – like the abundance of A. vaginae in disturbed vaginal microflora and the presence of several novel species – indicate that much is to be learned about the composition of the vaginal microflora and its relation to the etiology of BV.
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影响因子：
2.2
作者：
Baele, M;Vaneechoutte, M;Haesebrouck, F
通讯作者：
Haesebrouck, F
DOI：
10.1099/ijs.0.02441-0
发表时间：
2003-01-01
影响因子：
2.8
作者：
Hugenholtz, P;Huber, T
通讯作者：
Huber, T
影响因子：
3.6
作者：
Ison, CA;Hay, PE
通讯作者：
Hay, PE
影响因子：
4
作者：
Pavlova, SI;Kilic, AO;Tao, L
通讯作者：
Tao, L
影响因子：
2.1
作者：
COLLINS, MD;WALLBANKS, S
通讯作者：
WALLBANKS, S