Dopaminergic tone persistently regulates voltage-gated ion current densities through the D1R-PKA axis, RNA polymerase II transcription, RNAi, mTORC1, and translation.
Dopaminergic tone persistently regulates voltage-gated ion current densities through the D1R-PKA axis, RNA polymerase II transcription, RNAi, mTORC1, and translation.
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DOI：
10.3389/fncel.2014.00039
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发表时间：
2014
影响因子：
5.3
通讯作者：
Baro DJ
中科院分区：
文献类型：
--
作者：
Krenz WD;Parker AR;Rodgers EW;Baro DJ
长期内在和突触可塑性必须协调，以确保稳定性和灵活性的神经元回路。协调可以通过共享转导组件来实现。多巴胺（DA）是一个公认的参与者在许多形式的长期突触可塑性。最近的研究表明，DA也参与了活动依赖和非依赖形式的长期内在可塑性。我们先前研究了DA使能的长期内在可塑性在一个单一的确定的神经元。幽门外侧神经元（LP）是甲壳动物口胃神经系统（STNS）中幽门神经网络的一个组成部分。LP表达1型DA受体（D1 Rs）。5 nM DA的1 h水浴应用随后洗脱产生LP瞬时钾电流（IA）的最大电导（Gmax）的显著增加，其在DA应用开始后约4 h达到峰值;此外，如果神经元活动的变化伴随DA应用，则还观察到LP超极化激活电流（Ih）的持续增加。在这里，我们用药理学和肽抑制剂重复这些实验，以确定所涉及的细胞过程和信号蛋白。我们发现，DA诱导的离子电导的持续性活性非依赖性（IA）和活性依赖性（Ih）变化依赖于许多相同的元素，使长期突触可塑性，包括：D1 R-蛋白激酶A（PKA）轴，RNA聚合酶II转录，RNA干扰（RNAi），和雷帕霉素（mTOR）依赖性翻译的机制目标。我们将数据解释为增加强直性DA浓度增强了microRNA（s）（miR）的表达，导致未鉴定蛋白质的帽依赖性翻译增加。
Long-term intrinsic and synaptic plasticity must be coordinated to ensure stability and flexibility in neuronal circuits. Coordination might be achieved through shared transduction components. Dopamine (DA) is a well-established participant in many forms of long-term synaptic plasticity. Recent work indicates that DA is also involved in both activity-dependent and -independent forms of long-term intrinsic plasticity. We previously examined DA-enabled long-term intrinsic plasticity in a single identified neuron. The lateral pyloric (LP) neuron is a component of the pyloric network in the crustacean stomatogastric nervous system (STNS). LP expresses type 1 DA receptors (D1Rs). A 1 h bath application of 5 nM DA followed by washout produced a significant increase in the maximal conductance (Gmax) of the LP transient potassium current (IA) that peaked ~4 h after the start of DA application; furthermore, if a change in neuronal activity accompanied the DA application, then a persistent increase in the LP hyperpolarization activated current (Ih) was also observed. Here, we repeated these experiments with pharmacological and peptide inhibitors to determine the cellular processes and signaling proteins involved. We discovered that the persistent, DA-induced activity-independent (IA) and activity-dependent (Ih) changes in ionic conductances depended upon many of the same elements that enable long-term synaptic plasticity, including: the D1R-protein kinase A (PKA) axis, RNA polymerase II transcription, RNA interference (RNAi), and mechanistic target of rapamycin (mTOR)-dependent translation. We interpret the data to mean that increasing the tonic DA concentration enhances expression of a microRNA(s) (miRs), resulting in increased cap-dependent translation of an unidentified protein(s).
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DOI：
10.1523/jneurosci.3771-09.2010
发表时间：
2010-02-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Chen X;Shu S;Schwartz LC;Sun C;Kapur J;Bayliss DA
通讯作者：
Bayliss DA
影响因子：
5.3
作者：
Baro, DJ;Ayali, A;Harris-Warrick, RM
通讯作者：
Harris-Warrick, RM
影响因子：
64.5
作者：
Ashraf, SI;McLoon, AL;Kunes, S
通讯作者：
Kunes, S
DOI：
10.1073/pnas.1017576108
发表时间：
2011-07-12
影响因子：
11.1
作者：
Cohen, Jonathan E.;Lee, Philip R.;Fields, R. Douglas
通讯作者：
Fields, R. Douglas
影响因子：
5.3
作者：
Eacker SM;Dawson TM;Dawson VL
通讯作者：
Dawson VL