Five OsS40 Family Members Are Identified as Senescence-Related Genes in Rice by Reverse Genetics Approach.
Five OsS40 Family Members Are Identified as Senescence-Related Genes in Rice by Reverse Genetics Approach.
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通过反向遗传学方法鉴定出五个OsS40家族成员为水稻衰老相关基因
DOI：
10.3389/fpls.2021.701529
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zheng X
中科院分区：
文献类型：
--
作者：
Habiba;Xu J;Gad AG;Luo Y;Fan C;Uddin JBG;Ul Ain N;Huang C;Zhang Y;Miao Y;Zheng X
我们在前期的工作中共鉴定了16个水稻OsS 40基因，但其功能尚不清楚。在这项研究中，使用CRISPR/cas9基因编辑技术敲除了13个OsS 40成员。在筛选CRISPR/Cas9突变体与WT相比的表型表征后，5个oss 40 s突变体在抽穗后30天表现出持绿表型。此外，oss 40 -1、oss 40 -12和oss 40 -14的籽粒大小和粒重增加，而oss 40 -7和oss 40 -13的粒重下降。与野生型相比，在5个保绿oss 40 s突变体中，几个衰老相关基因（SAG）、叶绿素降解相关基因（CDG）以及WRKY成员的转录水平差异降低。5个oss 40突变体在离体叶片黑暗培养4天后也表现出持绿表型。与野生型相比，OsSWEET 4和OsSWEET 1b在Os 40 -7和Os 40 -14中显著上调，而OsSWEET 1a和OsSWEET 13在Os 40 -7和Os 40 -14中显著下调。此外，这5个OsS 40都具有较强的转录激活活性，并且定位于细胞核中。OsS 40基因在Os 40 -1、Os 40 -7和Os 40 -12突变体中表达下调，而在Os 40 -13和Os 40 -14突变体中表达上调，表明OsS 40基因之间存在协同调控。这些结果表明，OsS 40 -1，OsS 40 -7，OsS 40 -12，OsS 40 -13和OsS 40 -14是衰老相关基因，通过调节其他OsS 40成员，SWEET成员基因和衰老相关基因的表达参与衰老和碳分配网络。
A total of 16 OsS40 genes of Oryza sativa were identified in our previous work, but their functions remain unclear. In this study, 13 OsS40 members were knocked out using the CRISPR/cas9 gene-editing technology. After screening phenotype characterization of CRISPR/Cas9 mutants compared to WT, five oss40s mutants exhibited a stay-green phenotype at 30 days after heading. Moreover, increased grain size and grain weight occurred in the oss40-1, oss40-12, and oss40-14 lines, while declined grain weight appeared in the oss40-7 and oss40-13 mutants. The transcript levels of several senescence-associated genes (SAGs), chlorophyll degradation-related genes (CDGs), as well as WRKY members were differentially decreased in the five stay-green oss40s mutants compared to WT. Five oss40 mutants also exhibited a stay-green phenotype when the detached leaves were incubated under darkness for 4 days. OsSWEET4 and OsSWEET1b were significantly upregulated, while OsSWEET1a and OsSWEET13 were significantly downregulated in both oss40-7 and oss40-14 compared to WT. Furthermore, these five OsS40 displayed strong transcriptional activation activity and were located in the nucleus. Most of the OsS40 genes were downregulated in the oss40-1, oss40-7, and oss40-12 mutants, but upregulated in the oss40-13 and oss40-14 mutants, indicating coordinated regulation among OsS40 members. These results suggest that OsS40-1, OsS40-7, OsS40-12, OsS40-13, and OsS40-14 are senescence-associated genes, involved in the senescence and carbon allocation network by modulating other OsS40 members, SWEET member genes, and senescence-related gene expression.
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影响因子：
5.3
作者：
Fatima M;Ma X;Zhou P;Zaynab M;Ming R
通讯作者：
Ming R
影响因子：
9.4
作者：
Bezrutczyk, Margaret;Hartwig, Thomas;Sosso, Davide
通讯作者：
Sosso, Davide
DOI：
10.1007/978-1-62703-986-4_17
发表时间：
2014-01-01
期刊：
PLANT-PATHOGEN INTERACTIONS: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
作者：
Fraiture, Malou;Zheng, Xiangzi;Brunner, Frederic
通讯作者：
Brunner, Frederic
影响因子：
5.4
作者：
Cui, KH;Peng, SB;Zhang, Q
通讯作者：
Zhang, Q
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A