Metamorphosis‐dependent transcriptional regulation of xak‐c, a novel Xenopus type I keratin gene
Metamorphosis‐dependent transcriptional regulation of xak‐c, a novel Xenopus type I keratin gene
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xak-c（一种新型非洲爪蟾 I 型角蛋白基因）的变态依赖性转录调控
DOI：
10.1002/dvdy.10196
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发表时间：
2002
影响因子：
2.5
通讯作者：
K. Yoshizato
中科院分区：
文献类型：
--
作者：
Yusuke Watanabe;R. Tanaka;Hisao Kobayashi;R. Utoh;Ken;M. Obara;K. Yoshizato
无尾两栖类的幼虫在变态过程中将其表皮转化为成虫的表皮。这一过程的主要事件是幼虫表皮基底细胞的增殖和分化成成虫。本研究分离出了一种新的I型角蛋白cDNA，称为xak‐c（非洲爪蟾成体角蛋白c），它只在成体表皮基底细胞中表达。该基因在变态初期开始在幼虫表皮表达。甲状腺激素（TH）诱导该基因在变态前蝌蚪表皮中的早熟表达。为了研究该基因的转录调控与表皮变态的关系，克隆了xak-c基因5′侧翼区的一个2.8 kb片段，并对其启动子活性进行了研究。将xak-c启动子区域和作为报告基因的EGFP或荧光素酶的基因作为基因构建体，转染到各种细胞中，结果显示5′侧翼区域在无尾两栖动物和哺乳动物中均具有表皮细胞特异性转录活性。用构建体转染非洲爪蟾的幼虫皮肤组织，并在存在和不存在TH的情况下培养，这表明启动子区域对TH有响应，尽管该区域不包含共有TH响应元件样序列。与此形成鲜明对比的是，启动子区域对成年皮肤中的TH没有反应，这清楚地表明克隆的区域包含对变态依赖性转录因子有反应的特定序列。© 2002 Wiley利斯公司
Anuran larvae transform their epidermis to the adult counterpart during metamorphosis. The major event of this process is the proliferation of larval epidermal basal cells and their differentiation into adult ones. The present study isolated novel type I keratin cDNA dubbed xak‐c (Xenopus adult keratin‐c) that was exclusively expressed in adult epidermal basal cells. The gene started its expression in the larval epidermis at the onset of metamorphosis. Thyroid hormone (TH) induced the precocious expression of the gene in the epidermis of premetamorphic tadpoles. To study the transcriptional regulation of this gene in relation to epidermal metamorphosis, a 2.8 kb 5′‐flanking region of xak‐c was cloned and its promoter activity was investigated. Gene constructs were made so as to contain the xak‐c promoter region and gene of EGFP or luciferase as a reporter gene and were transfected into various types of cells, which revealed that the 5′‐flanking region had an epidermal cell‐specific transcriptional activity in both anurans and mammals. Larval skin tissues of Xenopus were transfected with the constructs and cultured in the presence and absence of TH, which showed that the promoter region is responsive to TH, although the region did not contain the consensus TH response element‐like sequence. In sharp contrast, the promoter region did not respond to TH in the adult skin, clearly indicating that the cloned region contains specific sequences that respond to metamorphosis‐dependent transcription factor(s). © 2002 Wiley‐Liss, Inc.
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非洲爪蟾发育过程中表皮角蛋白表达的空间、时间和激素调节。
影响因子：
2.7
作者：
Nishikawa,A;Shimizu-Nishikawa,K;Miller,L
通讯作者：
Miller,L
DOI：
10.1073/pnas.260141297
发表时间：
2000-11
影响因子：
11.1
作者：
Laurent M. Sachs;Yun-Bo Shi
通讯作者：
Laurent M. Sachs;Yun-Bo Shi
影响因子：
4.6
作者：
Carolyn Byrne;Michael Tainsky;Elaine Fuchs
通讯作者：
Carolyn Byrne;Michael Tainsky;Elaine Fuchs
变态过程中爪蟾 63 kDa 角蛋白基因启动子区域的组织特异性体内蛋白质-DNA 相互作用。
影响因子：
14.9
作者：
Warshawsky,D;Miller,L
通讯作者：
Miller,L
影响因子：
2.7
作者：
Luo, T;Matsuo-Takasaki, M;Sargent, TD
通讯作者：
Sargent, TD