Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk-Gelatin Composite Hydrogel Microbeads.
Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk-Gelatin Composite Hydrogel Microbeads.
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通过封装在藻酸盐模板上，双重交联的丝绸和丝绸明星蛋白复合水凝胶微粒中，人类祖细胞和干细胞的细胞保护作用。
DOI：
10.1002/adhm.202200293
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发表时间：
2022-09
影响因子：
10
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
--
作者：
Hasturk, Onur;Smiley, Jordan A.;Arnett, Miles;Sahoo, Jugal Kishore;Staii, Cristian;Kaplan, David L.
哺乳动物细胞对苛刻加工条件的敏感性限制了它们在细胞移植和组织工程应用中的应用。除了调节细胞微环境之外，将哺乳动物细胞包封在水凝胶微珠内通过物理隔离包封的细胞来引起细胞保护的注意。用于细胞微囊化的水凝胶制剂主要由离子交联的藻酸盐主导，其在生理培养条件下具有低的结构稳定性和差的细胞-基质相互作用。在这里，我们展示了藻酸盐模板的丝和丝/明胶复合水凝胶微球的永久性或按需裂解酶交联使用简单和具有成本效益的离心液滴处理的制造。复合微珠在离子交换条件下显示出结构稳定性，与离子交联的藻酸盐微球相比具有改善的机械性能。人间充质干细胞和神经祖细胞被成功地封装在复合珠中，并受到保护免受环境因素的影响，包括暴露于聚阳离子、细胞外酸中毒、凋亡细胞因子、紫外线照射、失巢凋亡、免疫识别，特别是机械应力。微珠通过27号细针在粘性聚环氧乙烷（PEO）溶液中挤出后保持封装的干细胞和祖细胞的活力、生长和分化，这表明在基于注射的递送和哺乳动物细胞的3D生物打印中具有更高的成功率。以海藻酸钠为模板，通过离心法制备了双交联的可降解蚕丝和蚕丝/明胶复合水凝胶微球。复合微珠显示出尺寸选择性渗透性，以及比仅海藻酸盐对照更好的机械性能和UV吸收。人干细胞和祖细胞被成功地封装在复合微珠中，对活力的影响最小，并在毛细血管流动期间免受抗体识别、聚阳离子、凋亡细胞因子、细胞外酸中毒、UV-C辐射和流体动力学的影响。
Susceptibility of mammalian cells against harsh processing conditions limit their use in cell transplantation and tissue engineering applications. Besides modulation of the cell microenvironment, encapsulation of mammalian cells within hydrogel microbeads attract attention for cytoprotection through physical isolation of the encapsulated cells. The hydrogel formulations used for cell microencapsulation are largely dominated by ionically crosslinked alginate, which suffer from low structural stability under physiological culture conditions and poor cell-matrix interactions. Here we demonstrate the fabrication of alginate templated silk and silk/gelatin composite hydrogel microspheres with permanent or on-demand cleavable enzymatic crosslinks using simple and cost-effective centrifugation-based droplet processing. Composite microbeads display structural stability under ion exchange conditions with improved mechanical properties compared to ionically crosslinked alginate microspheres. Human mesenchymal stem and neural progenitor cells are successfully encapsulated in the composite beads and protected against environmental factors, including exposure to polycations, extracellular acidosis, apoptotic cytokines, UV irradiation, anoikis, immune recognition, and particularly mechanical stress. Microbeads preserve viability, growth, and differentiation of encapsulated stem and progenitor cells after extrusion in viscous polyethylene oxide (PEO) solution through a 27-gauge fine needle, suggesting potential applications in injection-based delivery and 3D bioprinting of mammalian cells with higher success rates. Alginate templated, dual crosslinked on-demand degradable silk and silk/gelatin composite hydrogel microbeads are fabricated by centrifugation-based droplet formation. Composite microbeads display size-selective permeability, and better mechanical properties and UV absorption than alginate-only controls. Human stem and progenitor cells are successfully encapsulated in composite microbeads with minimum impact on viability, and protected against antibody recognition, polycations, apoptotic cytokine, extracellular acidosis, UV-C radiation, and hydrodynamic forces during capillary flow.
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影响因子：
3.8
作者：
BORN, C;ZHANG, Z;THOMAS, CR
通讯作者：
THOMAS, CR
使用间充质基质细胞的3D生物打印中的安全考虑。
影响因子：
5.7
作者：
Belk L;Tellisi N;Macdonald H;Erdem A;Ashammakhi N;Pountos I
通讯作者：
Pountos I
影响因子：
10.7
作者：
Davarci, Fatma;Turan, Deniz;Poncelet, Denis
通讯作者：
Poncelet, Denis
影响因子：
2.5
作者：
Ambasudhan, Rajesh;Dolatabadi, Nima;Nutter, Anthony;Masliah, Eliezer;McKercher, Scott R.;Lipton, Stuart A.
通讯作者：
Lipton, Stuart A.
影响因子：
82.9
作者：
Benedetti, S;Pirola, B;Finocchiaro, G
通讯作者：
Finocchiaro, G