In Situ Shape Control of Thermoplasmonic Gold Nanostars on Oxide Substrates for Hyperthermia-Mediated Cell Detachment.
In Situ Shape Control of Thermoplasmonic Gold Nanostars on Oxide Substrates for Hyperthermia-Mediated Cell Detachment.
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DOI：
10.1021/acscentsci.0c01097
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发表时间：
2020-11-25
影响因子：
18.2
通讯作者：
Jonas SJ
中科院分区：
文献类型：
--
作者：
Vinnacombe-Willson GA;Chiang N;Scarabelli L;Hu Y;Heidenreich LK;Li X;Gong Y;Inouye DT;Fisher TS;Weiss PS;Jonas SJ
金纳米星(AuNST)具有生物兼容性、大表面积和高近红外消光率的特点，使其成为与针对生物应用的技术集成的理想选择。我们开发了一种可靠而简单的微流控方法，用于在包括铟、锡氧化物和玻璃在内的氧化物衬底上直接生长各向异性的AuNSTs。优化了合成工艺，在间歇和微流控系统中制备出具有高各向异性、分支性、均匀性和密度的AuNSTs，以实现激光照射下最佳的光热转化。表面增强拉曼散射光谱和介观温度测量与空间相关扫描电子显微镜相结合，监测了不同激光强度下纳米STAR及其配体的稳定性和微泡的形成。利用该平台产生受控局部加热的能力，探索热疗辅助下移植到毛细血管壁上的黏附性胶质母细胞瘤细胞(U87-GFP)的脱落。流量和激光强度都可以调节以诱导不同的生物反应，如消融、细胞变形、细胞内成分释放和完整细胞的去除。最终，该平台在生物和化学传感、热疗介导的药物传递以及具有单细胞特异性的移植细胞的微流控软释放方面具有潜在的应用前景。在微流控通道中通过种子介导法原位生长的等离子体金纳米星被用来探测单个细胞，并将近红外光转化为热来选择性地释放它们。
Gold nanostars (AuNSTs) are biocompatible, have large surface areas, and are characterized by high near-infrared extinction, making them ideal for integration with technologies targeting biological applications. We have developed a robust and simple microfluidic method for the direct growth of anisotropic AuNSTs on oxide substrates including indium tin oxide and glass. The synthesis was optimized to yield AuNSTs with high anisotropy, branching, uniformity, and density in batch and microfluidic systems for optimal light-to-heat conversion upon laser irradiation. Surface-enhanced Raman scattering spectra and mesoscale temperature measurements were combined with spatially correlated scanning electron microscopy to monitor nanostar and ligand stability and microbubble formation at different laser fluences. The capability of the platform for generating controlled localized heating was used to explore hyperthermia-assisted detachment of adherent glioblastoma cells (U87-GFP) grafted to the capillary walls. Both flow and laser fluence can be tuned to induce different biological responses, such as ablation, cell deformation, release of intracellular components, and the removal of intact cells. Ultimately, this platform has potential applications in biological and chemical sensing, hyperthermia-mediated drug delivery, and microfluidic soft-release of grafted cells with single-cell specificity. Plasmonic gold nanostars grown in situ by seed-mediated synthesis in microfluidic channels are used to probe individual cells and to convert near-infrared light to heat to release them selectively.
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影响因子：
10.8
作者：
Hao, Feng;Nehl, Colleen L.;Nordlander, Peter
通讯作者：
Nordlander, Peter
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
DOI：
10.1073/pnas.1803884115
发表时间：
2018-10-02
影响因子：
11.1
作者：
Dhar, Manjima;Lam, Jeffrey Nam;Di Carlo, Dino
通讯作者：
Di Carlo, Dino
影响因子：
10
作者：
Espinosa, Ana;Silva, Amanda K. A.;Wilhelm, Claire
通讯作者：
Wilhelm, Claire
影响因子：
3.7
作者：
Ali, Moustafa R. K.;Wu, Yue;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.