Soluble E-cadherin fragments increased in circulation of cancer patients.
Soluble E-cadherin fragments increased in circulation of cancer patients.
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DOI：
10.1038/bjc.1994.106
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发表时间：
1994-03
影响因子：
8.8
通讯作者：
Kato, I.
中科院分区：
文献类型：
--
作者：
Katayama, M.;Hirai, S.;Kamihagi, K.;Nakagawa, K.;Yasumoto, M.;Kato, I.
针对人胎盘可溶性 E-钙粘蛋白产生单克隆抗体，并用于免疫酶测定法以检测生物体液中的可溶性 E-钙粘蛋白。 E-钙粘蛋白测定足够准确，可以定量细胞培养物上清液中可溶性E-钙粘蛋白的浓度。免疫反应性 E-钙粘蛋白被鉴定为以可溶形式存在于正常血清中，其分子量明显低于完整的 E-钙粘蛋白分子（约 80 kDa）。我们发现，与正常水平（1.99 +/- 0.50 微克 ml-1）相比，所研究的癌症患者血清中的免疫反应性 E-钙粘蛋白水平显着升高（平均值 +/- s.d. 3.80 +/- 2.36 微克 ml-1，P < 0.0001）。我们还发现，22 名胃癌患者（3.51 +/- 1.78 µg ml-1，P < 0.02）和 11 名肝细胞癌患者（5.55 +/- 3.11 µg ml-1，P < 0.001）血清 E-cadherin 水平显着高于 26 名糖尿病患者（2.33 +/- 1.58 µg）。毫升-1）。在 54 名癌症患者中，53.7% 的患者血清中可溶性 E-钙粘蛋白含量升高。因此，很明显，循环中的可溶性 E-钙粘蛋白可用作前瞻性肿瘤标志物，准确反映肿瘤部位 E-钙粘蛋白的渐进再生，这可能是由肿瘤相关的蛋白水解降解诱导的。
Monoclonal antibodies were raised against human placental soluble E-cadherins and used in an immunoenzymometric assay to detect soluble E-cadherins in biological fluids. The E-cadherin assay was accurate enough to quantitate the concentration of soluble E-cadherin in the cell culture supernatants. Immunoreactive E-cadherins, identified as existing in the soluble form in normal serum, were shown to have apparent lower molecular mass (approximately 80 kDa) than intact molecules of E-cadherin. We found that the immunoreactive E-cadherin levels in the serum of the studied cancer patients were significantly elevated (mean +/- s.d. 3.80 +/- 2.36 micrograms ml-1, P < 0.0001) when compared with the normal levels (1.99 +/- 0.50 micrograms ml-1). We also found that serum E-cadherin levels in the 22 patients with gastric cancer (3.51 +/- 1.78 micrograms ml-1, P < 0.02) or the 11 patients with hepatocellular cancer (5.55 +/- 3.11 micrograms ml-1, P < 0.001) were significantly higher than those in the 26 diabetic patients (2.33 +/- 1.58 micrograms ml-1). Of the 54 cancer patients, 53.7% exhibited an elevated amount of soluble E-cadherin in serum. Thus, it is evident that soluble E-cadherin in circulation can be used as a prospective tumour marker that accurately reflects the progressive regeneration of E-cadherin at tumour sites, potentially induced by tumour-associated proteolytic degradation.
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影响因子：
5.7
作者：
GOLDFARB, RH;LIOTTA, LA
通讯作者：
LIOTTA, LA
影响因子：
64.5
作者：
DAMSKY, CH;RICHA, J;BUCK, CA
通讯作者：
BUCK, CA
影响因子：
8.8
作者：
Matsuura, K;Kawanishi, J;Fujii, S;Imamura, M;Hirano, S;Takeichi, M;Niitsu, Y
通讯作者：
Niitsu, Y
DOI：
10.1083/jcb.115.2.517
发表时间：
1991-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Navarro P;Gómez M;Pizarro A;Gamallo C;Quintanilla M;Cano A
通讯作者：
Cano A
影响因子：
11.4
作者：
HAMAGUCHI, M;MATSUYOSHI, N;NAGAI, Y
通讯作者：
NAGAI, Y