Chlamydomonas: Intraflagellar Transport
Chlamydomonas: Intraflagellar Transport
复制标题
衣藻：鞭毛内运输
DOI：
10.1007/978-3-319-66360-9_5
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kaiyao Huang
中科院分区：
文献类型：
--
作者：
Gai Liu;Kaiyao Huang
纤毛/鞭毛是大多数真核细胞表面的微管细胞器，完成运动和感觉功能。纤毛功能障碍导致各种纤毛病，如多囊肾病、视网膜变性和男性不育症。几乎所有纤毛/鞭毛的组装都依赖于一种保守的转运机制-鞭毛内转运（IFT），IFT由22个亚基组成，由IFT-A和IFT-B复合体组成。IFT在鞭毛中双向运动，并由顺行运动驱动蛋白2和逆行运动动力蛋白1b/2驱动。IFT复合物的共表达和晶体结构分析表明，IFT-B可分为IFT-B1和IFT-B2两个亚复合物，每个亚复合物都有一个微管蛋白结合模块，提示微管蛋白是IFT的主要运载物。其他货物包括来自外动力蛋白臂（ODA）、内动力蛋白臂（IDA）、辐射辐蛋白（RSP）和动力蛋白调节复合物（DRC）的轴丝组分以及膜蛋白。电镜下鞭毛中的IFT和货物形成长串和短串。IFT通过转运鞭毛前体、周转产物或信号分子，在衣原体鞭毛组装、拆卸和信号转导中起着关键作用。IFT蛋白除了在鞭毛中移动外，还位于鞭毛的基部和顶端，是IFT调控的发生部位。未来的研究需要阐明IFT与货物之间的相互作用机制、IFT与鞭毛膜运输的关系、IFT在体内结构的复杂性和灵活性以及在体外重构IFT机制。
Cilia/flagella are microtubule-based organelles emanating from the surface of most eukaryotic cells and accomplish motile and sensory functions. Malfunction of cilia results in a variety of ciliopathies such as polycystic kidney disease, retinal degeneration, and male infertility. The assembly of nearly all cilia/flagella depends on a conserved transport machinery–intraflagellar transport (IFT), which has 22 subunits and is composed of IFT-A and IFT-B complex. IFT moves bidirectionally in flagella and is driven by the anterograde motor kinesin-2 and the retrograde motor dynein 1b/2. Co-expression and crystal structure analysis of IFT complex demonstrated that IFT-B can be divided into IFT-B1 and IFT-B2 subcomplex, and each subcomplex has a tubulin-binding module, suggesting that tubulin is a primary cargo of IFT. Other cargoes include axonemal components from outer dynein arms (ODA), inner dynein arms (IDA), radial spoke proteins (RSP) and dynein regulatory complexes (DRC), and membrane proteins. The IFT and cargoes form long trains and short trains in flagella under the electron microscopy. By transporting the flagellar precursors, turnover products, or signal molecules, IFT plays a critical role in flagellar assembly, disassembly, and signal transduction in Chlamydomonas. In addition to moving in flagella, IFT proteins also locate at the basal body and tip of flagella, where the regulation of IFT occurs. Future studies need to illustrate the interaction mechanism between the IFT and cargoes, relationship between IFT and the flagellar membrane trafficking, and the complexity and flexibility of the structure of IFT in vivo and to reconstitute the IFT machinery in vitro.
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影响因子：
9.2
作者：
Pedersen, LB;Gelmer, S;Rosenbaum, JL
通讯作者：
Rosenbaum, JL
影响因子：
3.3
作者：
Harris JA;Liu Y;Yang P;Kner P;Lechtreck KF
通讯作者：
Lechtreck KF
影响因子：
--
作者：
Lechtreck, Karl-Ferdinand;Luro, Scott;Awata, Junya;Witman, George B.
通讯作者：
Witman, George B.
研究纤毛内分子运动的方法。
DOI：
10.1007/978-1-4939-3789-9_6
发表时间：
2016
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Lechtreck KF
通讯作者：
Lechtreck KF
影响因子：
7.8
作者：
Pazour, GJ;Wilkerson, CG;Witman, GB
通讯作者：
Witman, GB