The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.
The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.
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DOI：
10.1038/s41598-018-25411-x
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发表时间：
2018-05-04
影响因子：
4.6
通讯作者：
Peck MW
中科院分区：
文献类型：
--
作者：
Brunt J;Carter AT;Pye HV;Peck MW
肉毒杆菌是一种厌氧孢子形成细菌，产生强效肉毒杆菌神经毒素，导致人类和动物的严重和致命的神经麻痹疾病（肉毒中毒）。C.肉毒杆菌II族是一种嗜冷性溶糖细菌，能形成中等耐热性的孢子，对最低限度加热的冷藏食品有特殊危害。孢子萌发是孢子向营养细胞过渡的基本过程，通常涉及对环境信号作出反应的发芽受体（GR）。对肉毒杆菌II群基因组的分析表明，它们包含一个单一的GR簇（gerX3b）和一个额外的单一gerA亚基（gerXAO）。肉毒杆菌II组菌株Eklund 17B孢子在添加l -丙氨酸后萌发，但在添加外源Ca2+-DPA后不萌发。该菌株的插入失活实验意外地揭示了孤儿GR GerXAO对l -丙氨酸刺激萌发至关重要。GerX3bA和GerX3bC对萌发率有影响，但在没有GerXAO的情况下不能诱导萌发。无法确定GerX3bB的作用。本研究首次鉴定了肉毒杆菌II群的功能性萌发受体。
Clostridium botulinum is an anaerobic spore forming bacterium that produces the potent botulinum neurotoxin that causes a severe and fatal neuro-paralytic disease of humans and animals (botulism). C. botulinum Group II is a psychrotrophic saccharolytic bacterium that forms spores of moderate heat resistance and is a particular hazard in minimally heated chilled foods. Spore germination is a fundamental process that allows the spore to transition to a vegetative cell and typically involves a germinant receptor (GR) that responds to environmental signals. Analysis of C. botulinum Group II genomes shows they contain a single GR cluster (gerX3b), and an additional single gerA subunit (gerXAO). Spores of C. botulinum Group II strain Eklund 17B germinated in response to the addition of L-alanine, but did not germinate following the addition of exogenous Ca2+-DPA. Insertional inactivation experiments in this strain unexpectedly revealed that the orphan GR GerXAO is essential for L-alanine stimulated germination. GerX3bA and GerX3bC affected the germination rate but were unable to induce germination in the absence of GerXAO. No role could be identified for GerX3bB. This is the first study to identify the functional germination receptor of C. botulinum Group II.
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影响因子：
5.2
作者：
Brunt J;van Vliet AH;van den Bos F;Carter AT;Peck MW
通讯作者：
Peck MW
影响因子：
4.4
作者：
Carter AT;Paul CJ;Mason DR;Twine SM;Alston MJ;Logan SM;Austin JW;Peck MW
通讯作者：
Peck MW
影响因子：
2.3
作者：
Broussolle, V;Alberto, F;Carlin, F
通讯作者：
Carlin, F
影响因子：
4.4
作者：
Leclair, Daniel;Farber, Jeffrey M.;Austin, John W.
通讯作者：
Austin, John W.
影响因子：
3.2
作者：
Cooper, Gareth R.;Moir, Anne
通讯作者：
Moir, Anne