Live cell imaging of intracellular Salmonella enterica.

Live cell imaging of intracellular Salmonella enterica.
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细胞内肠沙门氏菌的活细胞成像

DOI:
10.1007/978-1-4939-1625-2_13
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发表时间:
2014
影响因子:
--
通讯作者:
Hensel
Hensel
中科院分区:
--
文献类型:
--
作者:
Hensel

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在致病性生活方式的细胞内阶段,沙门氏菌会大量改变宿主细胞的内体系统。两个标志是吞噬体重塑为含沙门氏菌液泡(SCV)作为复制生态位,以及形成管状结构,如沙门氏菌诱导细丝(SIFs)。为了研究这些沙门氏菌特异性区室的动力学和命运,活细胞成像(LCI)是一种选择的方法。在本章中,我们比较了目前使用的显微镜技术,并重点讨论了LCI的特定考虑因素和要求。提供了用共聚焦激光扫描显微镜(CLSM)或旋转盘共聚焦显微镜(SDCM)检测沙门氏菌感染LCI的详细方案。
During the intracellular phase of the pathogenic lifestyle,Salmonella entericamassively alters the endosomal system of its host cells. Two hallmarks are the remodeling of phagosomes into theSalmonella-containing vacuole (SCV) as a replicative niche, and the formation of tubular structures, such asSalmonella-induced filaments (SIFs). To study the dynamics and the fate of theseSalmonella-specific compartments, live cell imaging (LCI) is a method of choice. In this chapter, we compare currently used microscopy techniques and focus on considerations and requirements specific for LCI. Detailed protocols for LCI ofSalmonellainfection with either confocal laser scanning microscopy (CLSM) or spinning disk confocal microscopy (SDCM) are provided.
DOI: 10.2144/000113765
发表时间: 2011-11
期刊: BioTechniques
影响因子: 2.7
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沙门氏菌在上皮细胞内的细胞内复制与含有溶酶体膜糖蛋白的丝状结构有关。
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