Edaravone, a free radical scavenger, mitigates both gray and white matter damages after global cerebral ischemia in rats.
Edaravone, a free radical scavenger, mitigates both gray and white matter damages after global cerebral ischemia in rats.
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DOI：
10.1016/j.brainres.2009.04.045
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发表时间：
2009-07-07
期刊：
影响因子：
2.9
通讯作者：
Shingu K
中科院分区：
文献类型：
--
作者：
Kubo K;Nakao S;Jomura S;Sakamoto S;Miyamoto E;Xu Y;Tomimoto H;Inada T;Shingu K
最近的研究表明，与大脑灰质（主要由神经元胞体组成）相似，白色物质（由轴突和胶质细胞组成）也易受缺血损伤。依达拉奉是一种自由基清除剂，即使在人类中也具有对抗局灶性脑缺血的神经保护作用。在这项研究中，我们研究了心脏骤停引起的全脑缺血后灰质和白色物质损伤的时间过程和严重程度，并检查依达拉奉是否可以保护灰质和白色物质。使用雄性Sprague-Dawley大鼠。通过心脏停搏和复苏（CAR）5分钟诱导全脑缺血。CAR后立即或60分钟静脉内给予依达拉奉3 mg/kg。甲酚紫染色观察细胞形态学变化。对微管相关蛋白2（神经元胞体和树突的标志物）、β淀粉样前体蛋白（其积累是轴突损伤的标志物）和离子钙结合衔接分子1（小胶质细胞的标志物）进行染色，以进行免疫组织化学分析。CAR后1周，在海马CA 1区观察到显著的神经元核周损伤和显著的小胶质细胞活化，几乎没有轴突损伤。CAR后2周，核周损伤和小胶质细胞活化无明显变化，但出现明显的轴突损伤。CAR后60分钟给予依达拉奉显著减轻了核周损伤、轴突损伤和小胶质细胞活化。我们的研究结果表明，轴突损伤的发展速度慢于核周损伤，依达拉奉可以保护大鼠CAR后的灰质和白色物质。
Recent studies have shown that similar to cerebral gray matter (mainly composed of neuronal perikarya), white matter (composed of axons and glias) is vulnerable to ischemia. Edaravone, a free radical scavenger, has neuroprotective effects against focal cerebral ischemia even in humans. In this study, we investigated the time course and the severity of both gray and white matter damage following global cerebral ischemia by cardiac arrest, and examined whether edaravone protected the gray and the white matter. Male Sprague-Dawley rats were used. Global cerebral ischemia was induced by 5 minutes of cardiac arrest and resuscitation (CAR). Edaravone, 3 mg/kg, was administered intravenously either immediately or 60 minutes after CAR. The morphological damage was assessed by cresyl violet staining. The microtubule-associated protein 2 (a maker of neuronal perikarya and dendrites), the β amyloid precursor protein (the accumulation of which is a maker of axonal damage), and the ionized calcium binding adaptor molecule 1 (a marker of microglia) were stained for immunohistochemical analysis. Significant neuronal perikaryal damage and marked microglial activation were observed in the hippocampal CA1 region with little axonal damage one week after CAR. Two weeks after CAR, the perikaryal damage and microglial activation were unchanged, but obvious axonal damage occurred. Administration of edaravone 60 minutes after CAR significantly mitigated the perikaryal damage, the axonal damage, and the microglial activation. Our results show that axonal damage develops slower than perikaryal damage and that edaravone can protect both gray and white matter after CAR in rats.
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影响因子：
5
作者：
Amemiya, S;Kamiya, T;Katayama, Y
通讯作者：
Katayama, Y
影响因子：
12.7
作者：
HORN, M;SCHLOTE, W
通讯作者：
SCHLOTE, W
影响因子：
8.3
作者：
Miyamoto, E;Tomimoto, H;Shingu, K
通讯作者：
Shingu, K
DOI：
10.1634/stemcells.2008-0075
发表时间：
2008-11
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Hirko AC;Dallasen R;Jomura S;Xu Y
通讯作者：
Xu Y
DOI：
10.1073/pnas.87.4.1561
发表时间：
1990-02-01
影响因子：
11.1
作者：
KOO, EH;SISODIA, SS;PRICE, DL
通讯作者：
PRICE, DL