Dynamic pattern of endothelial cell adhesion molecule expression in muscle and perineural vessels from patients with classic polyarteritis nodosa.
Dynamic pattern of endothelial cell adhesion molecule expression in muscle and perineural vessels from patients with classic polyarteritis nodosa.
复制标题
典型结节性多动脉炎患者肌肉和神经周围血管内皮细胞粘附分子表达的动态模式。
DOI：
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复制
发表时间：
1998
影响因子：
--
通讯作者：
Josep Maria Salanova Grau
中科院分区：
文献类型：
--
作者：
Blanca Coll;M. Cebrián;Maria C. Cid;Carme Font;Jordi Esparza;Manel Juan;Jordi Yagüe;Alvaro Urbano;Josep Maria Salanova Grau
客观化
探讨经典型结节性多动脉炎(PAN)患者血管内皮细胞黏附分子的表达。
方法
应用识别黏附分子的特异性单抗，对30例经活检证实的PAN患者的21块肌肉和16条神经标本、12例组织学正常肌肉和2例正常神经标本进行了免疫组织化学研究。黏附分子包括细胞间黏附分子1、细胞间黏附分子2、细胞间黏附分子3、血管细胞黏附分子1、血小板内皮细胞黏附分子1、E选择素、P选择素、L选择素、淋巴细胞功能相关抗原1和极晚期激活抗原4。用识别中性粒细胞弹性蛋白酶的单抗鉴定中性粒细胞。用凝集素Ulex uropaeus鉴定内皮细胞。
结果
在早期病变中，PECAM-1、ICAM-1、ICAM-2和P-选择素的表达与对照组相似，血管内皮细胞可诱导VCAM-1和E-选择素的表达。在晚期病变中，黏附分子的免疫染色在管腔内皮细胞中减少或消失，而这些分子在炎症血管内和周围的微血管中明显表达。愈合阶段的血管内皮细胞染色呈阴性。高比例的浸润性白细胞表达LFA-1和VLA-4，只有少数表达L-选择素。未观察到黏附分子的表达模式与临床特征、病程或既往皮质类固醇治疗之间的关系。
结论
血管内皮细胞黏附分子在PAN中的表达是一个动态的过程，它随血管病变的组织病理分期而变化。PAN中结构性和诱导性黏附分子在微血管中的优先表达提示，血管生成有助于PAN炎症的持续渗透。
OBJECTIVE
To investigate endothelial cell adhesion molecule expression in vessels from patients with classic polyarteritis nodosa (PAN).
METHODS
Frozen sections of 21 muscle and 16 nerve samples from 30 patients with biopsy-proven PAN and 12 histologically normal muscle and 2 histologically normal nerve samples from 12 controls were studied immunohistochemically, using specific monoclonal antibodies (MAb) that recognize adhesion molecules. Adhesion molecules identified were intercellular adhesion molecule 1 (ICAM-1), ICAM-2, ICAM-3, vascular cell adhesion molecule 1 (VCAM-1), platelet endothelial cell adhesion molecule 1 (PECAM-1), E-selectin, P-selectin, L-selectin, lymphocyte function-associated antigen 1 (LFA-1), and very late activation antigen 4 (VLA-4). Neutrophils were identified with a MAb recognizing neutrophil elastase. Endothelial cells were identified with the lectin ulex europaeus.
RESULTS
In early lesions, expression of PECAM-1, ICAM-1, ICAM-2, and P-selectin was similar to that in control samples, and VCAM-1 and E-selectin were induced in vascular endothelium. In advanced lesions, immunostaining for adhesion molecules diminished or disappeared in luminal endothelium, whereas these molecules were clearly expressed in microvessels within and surrounding inflamed vessels. Staining in endothelia from vessels in a healing stage tended to be negative. A high proportion of infiltrating leukocytes expressed LFA-1 and VLA-4, and only a minority expressed L-selectin. No relationship between the expression pattern of adhesion molecules and clinical features, disease duration, or previous corticosteroid treatment was observed.
CONCLUSION
Endothelial adhesion molecule expression in PAN is a dynamic process that varies according to the histopathologic stage of the vascular lesions. The preferential expression of constitutive and inducible adhesion molecules in microvessels suggests that angiogenesis contributes to the persistence of inflammatory infiltration in PAN.
影响因子：
20.3
作者：
T. Carlos;J. Harlan
通讯作者：
T. Carlos;J. Harlan
DOI：
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发表时间：
1992-12
期刊：
The American journal of pathology
影响因子：
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作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
通讯作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
两种不同的单核因子，白细胞介素 1 和肿瘤坏死因子，各自独立诱导相同抗原在培养的人血管内皮细胞表面的生物合成和瞬时表达。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Pober,JS;Bevilacqua,MP;Mendrick,DL;Lapierre,LA;Fiers,W;GimbroneJr,MA
通讯作者：
GimbroneJr,MA