RNA-Seq analysis of a soybean near-isogenic line carrying bacterial leaf pustule-resistant and -susceptible alleles.
RNA-Seq analysis of a soybean near-isogenic line carrying bacterial leaf pustule-resistant and -susceptible alleles.
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DOI：
10.1093/dnares/dsr033
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Lee SH
中科院分区：
文献类型：
--
作者：
Kim KH;Kang YJ;Kim DH;Yoon MY;Moon JK;Kim MY;Van K;Lee SH
细菌性叶疱病（BLP）是由轴索黄单胞菌引起的疾病。甘氨酸(Xag)。为了研究Xag诱导的植物基础防御机制，采用RNA-Seq技术对大豆近等基因系（NILs）中blp敏感系和blp抗性系的差异基因表达进行了分析。在定位到大豆基因组参考序列的46 367个基因中，分别有1978个和783个基因在接种后0、6和12小时（hai）在抗blp的NIL中相对于blp敏感的NIL中表达上调和下调。聚类分析结果表明，这些基因可分为10个不同表达模式的聚类。基于基因本体（GO）分类进行了功能标注。在已鉴定的大豆防御应答基因（GO:0006952）中，134个基因在抗blp和敏感NILs之间的表达存在显著差异。特别是，病原菌相关分子模式（PAMP）和损伤相关分子模式（DAMP）受体及其诱导的基因在抗blp的NIL中在0位点高表达。另外，发病相关基因PR -1和-14在0 hai高表达，PR-3、-6和-12在12 hai高表达。核心ja信号元件MYC2和JASMONATE ZIM-motif的表达也存在显著差异。这些结果表明，参与识别PAMPs或DAMPs的强大基础防御机制以及防御相关基因产物的高水平积累可能有助于大豆对BLP的抗性。
Bacterial leaf pustule (BLP) disease is caused by Xanthomonas axonopodis pv. glycines (Xag). To investigate the plant basal defence mechanisms induced in response to Xag, differential gene expression in near-isogenic lines (NILs) of BLP-susceptible and BLP-resistant soybean was analysed by RNA-Seq. Of a total of 46 367 genes that were mapped to soybean genome reference sequences, 1978 and 783 genes were found to be up- and down-regulated, respectively, in the BLP-resistant NIL relative to the BLP-susceptible NIL at 0, 6, and 12h after inoculation (hai). Clustering analysis revealed that these genes could be grouped into 10 clusters with different expression patterns. Functional annotation based on gene ontology (GO) categories was carried out. Among the putative soybean defence response genes identified (GO:0006952), 134 exhibited significant differences in expression between the BLP-resistant and -susceptible NILs. In particular, pathogen-associated molecular pattern (PAMP) and damage-associated molecular pattern (DAMP) receptors and the genes induced by these receptors were highly expressed at 0 hai in the BLP-resistant NIL. Additionally, pathogenesis-related (PR)-1 and -14 were highly expressed at 0 hai, and PR-3, -6, and -12 were highly expressed at 12 hai. There were also significant differences in the expression of the core JA-signalling components MYC2 and JASMONATE ZIM-motif. These results indicate that powerful basal defence mechanisms involved in the recognition of PAMPs or DAMPs and a high level of accumulation of defence-related gene products may contribute to BLP resistance in soybean.
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影响因子：
11.6
作者：
Bieri, S;Mauch, S;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P
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Escobar, Carolina
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Koornneef, Maarten