Supramolecular regulation of bioorthogonal catalysis in cells using nanoparticle-embedded transition metal catalysts.
Supramolecular regulation of bioorthogonal catalysis in cells using nanoparticle-embedded transition metal catalysts.
复制标题
DOI：
10.1038/nchem.2284
复制
发表时间：
2015-07
期刊：
影响因子：
21.8
通讯作者：
Rotello VM
中科院分区：
文献类型：
--
作者：
Tonga GY;Jeong Y;Duncan B;Mizuhara T;Mout R;Das R;Kim ST;Yeh YC;Yan B;Hou S;Rotello VM
生物正交催化拓宽了细胞内化学的功能可能性。由于复杂的细胞内环境和催化剂不稳定性，合成催化系统在细胞中的有效递送和调节具有挑战性。在这里，我们报告的蛋白质大小的生物正交纳米酶的制造通过封装到单层的水溶性金纳米粒子的疏水过渡金属催化剂。这些催化剂的活性可以通过将超分子葫芦[7]脲“看门人”结合到单层表面上来可逆地控制，从而提供模拟酶的变构调节的仿生控制机制。这种门控纳米酶用于成像和治疗应用的潜力通过触发切割烯丙基氨基甲酸酯用于前荧光团活化和炔丙基用于活细胞内的前药活化来证明。由于复杂的细胞内环境，生物正交催化在生命系统中的调节是具有挑战性的。现在，蛋白质大小的生物正交纳米酶的活性已经通过将超分子葫芦[7]脲“看门人”结合到单层表面上来调节，从而提供活细胞内前荧光团和前药的受控激活，用于成像和治疗应用。
Bioorthogonal catalysis broadens the functional possibilities of intracellular chemistry. Effective delivery and regulation of synthetic catalytic systems in cells is challenging due to the complex intracellular environment and catalyst instability. Here, we report the fabrication of protein-sized bioorthogonal nanozymes through the encapsulation of hydrophobic transition metal catalysts into the monolayer of water-soluble gold nanoparticles. The activity of these catalysts can be reversibly controlled by binding a supramolecular cucurbit[7]uril ‘gate-keeper’ onto the monolayer surface, providing a biomimetic control mechanism that mimics the allosteric regulation of enzymes. The potential of this gated nanozyme for use in imaging and therapeutic applications was demonstrated through triggered cleavage of allylcarbamates for pro-fluorophore activation and propargyl groups for prodrug activation inside living cells. Regulation of bioorthogonal catalysis in living systems is challenging because of the complex intracellular environment. Now, the activity of protein-sized bioorthogonal nanozymes has been regulated by binding a supramolecular cucurbit[7]uril ‘gate-keeper’ onto the monolayer surface, providing controlled activation of pro-fluorophores and prodrugs inside living cells for imaging and therapeutic applications.
登录
查看更多内容
影响因子：
29.4
作者：
Kim, Hyehyeon;Kim, Saehee;Kim, Chulhee
通讯作者：
Kim, Chulhee
影响因子：
2.7
作者：
Cai, SX;Zhang, HZ;Weber, E
通讯作者：
Weber, E
影响因子：
4.3
作者：
Alcaide, B;Almendros, P;Alonso, JM
通讯作者：
Alonso, JM
DOI：
10.1073/pnas.1113466109
发表时间：
2012-03-27
影响因子：
11.1
作者：
Devaraj, Neal K.;Thurber, Greg M.;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
21.8
作者：
Kim, Chaekyu;Agasti, Sarit S.;Zhu, Zhengjiang;Isaacs, Lyle;Rotello, Vincent M.
通讯作者：
Rotello, Vincent M.