The cell cycle, autophagy, and cell wall integrity pathway jointly governed by MoSwe1 in Magnaporthe oryzae.
The cell cycle, autophagy, and cell wall integrity pathway jointly governed by MoSwe1 in Magnaporthe oryzae.
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DOI：
10.1186/s12964-023-01389-6
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发表时间：
2024-01-09
影响因子：
8.4
通讯作者：
Lin, Fu-Cheng
中科院分区：
文献类型：
--
作者：
Li, Lin;Zhu, Xue-Ming;Bao, Jian-Dong;Wang, Jiao-Yu;Liu, Xiao-Hong;Lin, Fu-Cheng
细胞周期是植物病原真菌细胞分化的关键。细胞壁完整性（CWI）信号在应对细胞壁应激中起着至关重要的作用。自噬是细胞在应激条件下分解其组分以恢复大分子并提供能量的降解过程。然而，细胞周期，自噬和CWI途径之间的具体联系仍然不清楚在模式病原真菌Magnaporthe pasta。在这里，我们已经确定MoSwe 1作为保守的组成部分，在稻瘟病菌的细胞周期。我们已经发现MoSwe 1通过蛋白磷酸化作用靶向MoMps 1，MoMps 1是CWI通路中保守的关键MAP激酶，其正调节CWI信号传导。CWI通路在Δ Moswe 1突变体中异常，伴有细胞周期阻滞。此外，我们提供的证据表明，MoSwe 1正调控自噬与MoAtg 17和MoAtg 18，核心自噬蛋白相互作用。Δ Moswe 1突变体的S期启动提前，分生孢子和附着胞形态异常，隔膜形成和糖原降解受到影响。我们的研究确定MoSWE 1对G1/S转换、CWI途径和自噬的调节支持其在植物病原真菌中对附着胞发育和毒力的特定需求。视频摘要在线版本包含补充材料，可通过10. 1186/s12964-023-01389-6获取。
The cell cycle is pivotal to cellular differentiation in plant pathogenic fungi. Cell wall integrity (CWI) signaling plays an essential role in coping with cell wall stress. Autophagy is a degradation process in which cells decompose their components to recover macromolecules and provide energy under stress conditions. However, the specific association between cell cycle, autophagy and CWI pathway remains unclear in model pathogenic fungi Magnaporthe oryzae. Here, we have identified MoSwe1 as the conserved component of the cell cycle in the rice blast fungus. We have found that MoSwe1 targets MoMps1, a conserved critical MAP kinase of the CWI pathway, through protein phosphorylation that positively regulates CWI signaling. The CWI pathway is abnormal in the ΔMoswe1 mutant with cell cycle arrest. In addition, we provided evidence that MoSwe1 positively regulates autophagy by interacting with MoAtg17 and MoAtg18, the core autophagy proteins. Moreover, the S phase initiation was earlier, the morphology of conidia and appressoria was abnormal, and septum formation and glycogen degradation were impaired in the ΔMoswe1 mutant. Our research defines that MoSWE1 regulation of G1/S transition, CWI pathway, and autophagy supports its specific requirement for appressorium development and virulence in plant pathogenic fungi. Video Abstract The online version contains supplementary material available at 10.1186/s12964-023-01389-6.
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DOI：
10.1083/jcb.143.3.709
发表时间：
1998-11-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carroll CW;Altman R;Schieltz D;Yates JR;Kellogg D
通讯作者：
Kellogg D
影响因子：
3.5
作者：
Jeon, Junhyun;Goh, Jaeduk;Lee, Yong-Hwan
通讯作者：
Lee, Yong-Hwan
影响因子：
56.9
作者：
Dagdas, Yasin F.;Yoshino, Kae;Talbot, Nicholas J.
通讯作者：
Talbot, Nicholas J.
DOI：
10.1016/j.ijporl.2021.110751
发表时间：
2021-05-05
影响因子：
1.5
作者：
Gedik-Soyuyuce, Ozlem;Gence-Gumus, Zeynep;Korkut, Nazim
通讯作者：
Korkut, Nazim
影响因子：
1.7
作者：
Balzanelli C;Spataro D;Redaelli de Zinis LO
通讯作者：
Redaelli de Zinis LO