The molecular mechanism for activating IgA production by Pediococcus acidilactici K15 and the clinical impact in a randomized trial.
The molecular mechanism for activating IgA production by Pediococcus acidilactici K15 and the clinical impact in a randomized trial.
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DOI：
10.1038/s41598-018-23404-4
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发表时间：
2018-03-22
影响因子：
4.6
通讯作者：
Tsuji NM
中科院分区：
文献类型：
--
作者：
Kawashima T;Ikari N;Kouchi T;Kowatari Y;Kubota Y;Shimojo N;Tsuji NM
粘膜部位的伊加分泌对于宿主防御病原体以及维持与小肠中存在的影响伊加产生的微生物的共生关系是重要的。在本研究中，我们测试了5株乳酸菌刺激伊加产生的能力，选择乳酸片球菌K15作为诱导这种保护性免疫球蛋白最有效的菌株。我们发现，这种反应主要是通过IL-10诱导的，与IL-6一样有效，由K15刺激的树突状细胞分泌。此外，细菌RNA主要负责这些细胞因子的诱导;双链RNA是IL-6产生的主要致病分子，而单链RNA是IL-10产生的关键因素。在一项随机、双盲、安慰剂对照的临床试验中，与干预前观察到的基础水平相比，摄入K15显著增加了唾液中的分泌型伊加（sIgA）浓度。这些结果表明，功能性乳酸菌诱导树突状细胞产生IL-6和IL-10，这有助于上调人粘膜部位的sIgA浓度。
IgA secretion at mucosal sites is important for host defence against pathogens as well as maintaining the symbiosis with microorganisms present in the small intestine that affect IgA production. In the present study, we tested the ability of 5 strains of lactic acid bacteria stimulating IgA production, being Pediococcus acidilactici K15 selected as the most effective on inducing this protective immunoglobulin. We found that this response was mainly induced via IL-10, as efficiently as IL-6, secreted by K15-stimulated dendritic cells. Furthermore, bacterial RNA was largely responsible for the induction of these cytokines; double-stranded RNA was a major causative molecule for IL-6 production whereas single-stranded RNA was critical factor for IL-10 production. In a randomized, double-blind, placebo-controlled clinical trial, ingestion of K15 significantly increased the secretory IgA (sIgA) concentration in saliva compared with the basal level observed before this intervention. These results indicate that functional lactic acid bacteria induce IL-6 and IL-10 production by dendritic cells, which contribute to upregulating the sIgA concentration at mucosal sites in humans.
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影响因子：
30.3
作者：
Kubinak JL;Petersen C;Stephens WZ;Soto R;Bake E;O'Connell RM;Round JL
通讯作者：
Round JL
影响因子：
3.7
作者：
Schulte BM;Gielen PR;Kers-Rebel ED;Schreibelt G;van Kuppeveld FJ;Adema GJ
通讯作者：
Adema GJ
源自乳酸细菌的双链RNA通过人树突状细胞增强了Th1免疫力。
影响因子：
7.3
作者：
Kawashima T;Ikari N;Watanabe Y;Kubota Y;Yoshio S;Kanto T;Motohashi S;Shimojo N;Tsuji NM
通讯作者：
Tsuji NM
影响因子：
5.6
作者：
Maeda, Naoyoshi;Nakamura, Risa;Yoshikai, Yasunobu
通讯作者：
Yoshikai, Yasunobu
TACI和BAFF-R介导B细胞中的同种型切换。
影响因子：
15.3
作者：
Castigli, E;Wilson, SA;Scott, S;Dedeoglu, F;Xu, SL;Lam, KP;Bram, RJ;Jabara, H;Geha, RS
通讯作者：
Geha, RS