The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.
The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.
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DOI：
10.1016/j.jmb.2016.08.004
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发表时间：
2016-10-09
影响因子：
5.6
通讯作者：
Wigneshweraraj, S.
中科院分区：
文献类型：
--
作者：
Brown, D. R.;Sheppard, C. M.;Burchell, L.;Matthews, S.;Wigneshweraraj, S.
σ因子是细菌转录机器RNA聚合酶的功能性强制性亚基。噬菌体编码的调节或抑制细菌RNAP以允许噬菌体基因表达的时间调节的小蛋白通常靶向主要细菌σ因子σ70的活性。先前，我们表明，在黄单胞菌噬菌体Xp 10感染期间，噬菌体蛋白P7通过阻止与启动子的生产性接合来抑制宿主RNAP，同时从RNAP中置换σ70因子。在这项研究中，我们证明P7还抑制含有主要变体细菌σ因子σ54的细菌RNAP与其关联启动子的生产性接合。结果首次表明宿主RNAP的主要变体形式也可以被噬菌体编码的转录调节蛋白靶向。由于RNAP中的主要和主要变体σ因子相互作用表面基本上重叠，但σ70和σ54的不同区域用于结合RNAP，我们的结果进一步强调了σ-RNAP界面在细菌RNAP功能和调节中的重要性，并可能用于抗菌剂的干预。Xp 10噬菌体转录调节因子P7抑制含有σ54的RNAP的转录。P7阻止σ54-RNAP与启动子DNA的生产性接合。· P7破坏预先形成的σ54-RNAP-启动子复合物。
The σ factor is a functionally obligatory subunit of the bacterial transcription machinery, the RNA polymerase. Bacteriophage-encoded small proteins that either modulate or inhibit the bacterial RNAP to allow the temporal regulation of bacteriophage gene expression often target the activity of the major bacterial σ factor, σ70. Previously, we showed that during Xanthomonas oryzae phage Xp10 infection, the phage protein P7 inhibits the host RNAP by preventing the productive engagement with the promoter and simultaneously displaces the σ70 factor from the RNAP. In this study, we demonstrate that P7 also inhibits the productive engagement of the bacterial RNAP containing the major variant bacterial σ factor, σ54, with its cognate promoter. The results suggest for the first time that the major variant form of the host RNAP can also be targeted by bacteriophage-encoded transcription regulatory proteins. Since the major and major variant σ factor interacting surfaces in the RNAP substantially overlap, but different regions of σ70 and σ54 are used for binding to the RNAP, our results further underscore the importance of the σ–RNAP interface in bacterial RNAP function and regulation and potentially for intervention by antibacterials. Graphical abstract Xp10 phage transcription regulator P7 inhibits transcription by RNAP containing σ54. P7 prevents the productive engagement of the σ54–RNAP with the promoter DNA. • P7 disrupts preformed σ54–RNAP-promoter complexes.
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影响因子：
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作者：
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