DNA methylation restrains transposons from adopting a chromatin signature permissive for meiotic recombination.
DNA methylation restrains transposons from adopting a chromatin signature permissive for meiotic recombination.
复制标题
DOI：
10.1101/gad.257840.114
复制
发表时间：
2015-06-15
影响因子：
10.5
通讯作者：
Bourc'his D
中科院分区：
文献类型：
--
作者：
Zamudio N;Barau J;Teissandier A;Walter M;Borsos M;Servant N;Bourc'his D
Zamudio等人。证明DNA甲基化抑制转座子采用染色质特性服从减数分裂重组，他们认为这防止了不稳定的染色体事件的发生。DNA甲基化对于保护哺乳动物生殖系免受转座子的侵害是必不可少的。当基于DNA甲基化的转座子控制有缺陷时，减数分裂染色体配对在精子发生过程中一直受到损害：减数分裂如何以及为什么容易受到转座子活性的影响尚不清楚。使用两个DNA甲基化缺陷的背景，Dnmt3L和Miwi2突变小鼠，我们揭示了DNA甲基化在很大程度上是在减数分裂开始之前沉默转座子所必需的。在此之后，备份到发展程序化的H3K9me2损失变得至关重要。转座子去抑制后不会发生大规模的逆转录转座，但减数分裂的染色质景观受到了深刻的影响。的确，在转录活性转座子上获得的H3K4me3标记与DNMT3L−/−减数分裂细胞中依赖于SPO11的双链断裂的形成和DMC1修复酶的募集有关，而这些特征通常是减数分裂重组热点所独有的。在这里，我们证明了DNA甲基化抑制转座子采用染色质特性服从减数分裂重组，我们认为这防止了不规律的染色体事件的发生。
Zamudio et al. demonstrate that DNA methylation restrains transposons from adopting chromatin characteristics amenable to meiotic recombination, which they propose prevents the occurrence of erratic chromosomal events. DNA methylation is essential for protecting the mammalian germline against transposons. When DNA methylation-based transposon control is defective, meiotic chromosome pairing is consistently impaired during spermatogenesis: How and why meiosis is vulnerable to transposon activity is unknown. Using two DNA methylation-deficient backgrounds, the Dnmt3L and Miwi2 mutant mice, we reveal that DNA methylation is largely dispensable for silencing transposons before meiosis onset. After this, it becomes crucial to back up to a developmentally programmed H3K9me2 loss. Massive retrotransposition does not occur following transposon derepression, but the meiotic chromatin landscape is profoundly affected. Indeed, H3K4me3 marks gained over transcriptionally active transposons correlate with formation of SPO11-dependent double-strand breaks and recruitment of the DMC1 repair enzyme in Dnmt3L−/− meiotic cells, whereas these features are normally exclusive to meiotic recombination hot spots. Here, we demonstrate that DNA methylation restrains transposons from adopting chromatin characteristics amenable to meiotic recombination, which we propose prevents the occurrence of erratic chromosomal events.
登录
查看更多内容
影响因子：
6.4
作者：
Daskalos, Alexandros;Nikolaidis, Georgios;Liloglou, Triantafillos
通讯作者：
Liloglou, Triantafillos
影响因子：
10.5
作者：
Aravin, Alexei A.;Bourc'his, Deborah
通讯作者：
Bourc'his, Deborah
影响因子：
7
作者：
Baker CL;Walker M;Kajita S;Petkov PM;Paigen K
通讯作者：
Paigen K
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1530/jrf.0.1160379
发表时间：
1999-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Anderson, R;Schaible, K;Wylie, C
通讯作者：
Wylie, C