RUI: Design and Characterization of Immobilized Enzyme Stringed-Bead Reactors

RUI：固定化酶串珠反应器的设计和表征

• 批准号: 8412299
• 负责人: Robert Thompson
• 金额: $ 3.71万
• 依托单位: Oberlin College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8412299&HistoricalAwards=false
• 关键词: RUI; Design; Characterization; Immobilized; Enzyme

The goal of this research is to prepare and characterize single-bead-string reactors (SBSR's) in which enzymes have been immobilized. These reactors are prepared by packing a tube with solid glass spheres whose diameter is slightly larger than the radius of the tubing, creating a zig-zag pattern when properly packed. Two reactor designs will be examined, one with enzymes bonded to the tube walls and the other with enzymes immobilized on the beads. These immobilized enzyme systems will then be used to study mixing in continuous flow systems. Using a very active enzyme, such that molecular movement is the limiting factor (i.e., the rate at which the reactant reaches the enzyme), mass transfer can be calculated by measuring the changes in product concentration.

这项研究的目的是准备和表征 单珠串反应器（SBSR），其中酶已经 无法动弹 这些反应器是通过用固体填充管来制备的。 玻璃球的直径略大于半径的 管，创建一个锯齿形图案时，适当包装。 两个反应器 设计将被检查，一个与酶结合到管壁， 另一种是固定在珠子上的酶。 这些固定的 酶系统将用于研究连续流中的混合 系统. 使用非常活跃的酶，这样分子运动 限制因素（即，反应物到达反应器的速率 酶），质量传递可以通过测量 产品浓度。