Gas Chromatography - Mass Spectrometry Throughout the Chemistry Curriculum

气相色谱-质谱分析贯穿整个化学课程

• 批准号: 0088173
• 负责人: Robert Thompson
• 金额: $ 4.18万
• 依托单位: Oberlin College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0088173&HistoricalAwards=false
• 关键词: Gas; Chromatography; Mass; Spectrometry; Throughout

Chemistry (12) Mass spectrometry (MS) has become one of the premier techniques for the identificationof molecules and macromolecules. Recent developments in MS instrumentation and interfaces have ushered in a new era of chemical analysis that demands that scientists have knowledge of and experience with MS. While gas chromatography-mass spectrometry (GC-MS) is one of the oldest of the hyphenated techniques, it remains one of the better and most cost-effective means for teaching mass spectrometry to undergraduates. This acquisition is allowing the introduction of MS at all levels of the curriculum and to offer experiences in MS never before possible in our program. The new instrument has the following crucial features: (1) an attached autosampler to provide unattended analysis of samples; (2) access to chemical ionization to provide beginning students with less complex mass spectra; i.e., those that contain intense "molecular ion" peaks for easier molecular weight determination; and (3) an ion trap mass analyzer rather than a quadrupole that makes possible trace analysis experiments that depend upon the selectivity and sensitivity provided by GC-MS-MS.Guided inquiry and discovery-based experiments involving GC-MS that had goodsuccess at other institutions are being adapted or directly implemented into six courses in the curriculum by four of our eight full-time chemistry faculty. Students' knowledge of mass spectrometry is evolving from simple molecular weight information, introduced in general chemistry, to qualitative and quantitative analysis in the organic and analytical chemistry core courses, to analysis of fragmentation patterns and tandem mass spectrometry experienced in two advanced laboratory courses. Our research efforts with undergraduate students are being enhanced as well. This project is supporting major changes in our local general chemistry program and that may serve globally as a model for the integration of both routine and advanced applications of GC-MS in an undergraduate chemistry curriculum.

化学（12）质谱（MS）已成为鉴定分子和大分子的主要技术之一。MS仪器和接口的最新发展迎来了化学分析的新时代，要求科学家拥有MS的知识和经验。虽然气相色谱-质谱（GC-MS）是最古老的联用技术之一，但它仍然是向本科生教授质谱的最佳和最具成本效益的手段之一。此次收购允许在课程的各个层面引入MS，并提供MS的经验，这在我们的课程中是前所未有的。新仪器具有以下重要特点：（1）一个附加的自动进样器，提供无人值守的样品分析;（2）化学电离，为初学者提供不太复杂的质谱;即，那些含有强烈的“分子离子”峰，更容易测定分子量;和（3）离子阱质量分析器而不是四极杆，其使得可能的痕量分析实验取决于GC-MS-MS提供的选择性和灵敏度。MS在其他机构取得了很好的成功，我们的八个全职化学教师中有四个正在改编或直接实施到课程的六门课程中。学生的质谱知识正在从普通化学中介绍的简单分子量信息发展到有机和分析化学核心课程中的定性和定量分析，再到两门高级实验室课程中经历的碎片模式和串联质谱分析。我们与本科生的研究工作也在加强。该项目支持我们当地普通化学课程的重大变化，并可在全球范围内作为本科化学课程中常规和高级GC-MS应用整合的典范。