Evaporative Heat Moisture Control in Solid Substrate Fermentation of Wheat Straw for Cellulase Production

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The objective of this research is to develop an automated, computerized and integrated evaporative heat-moisture control system in the solid substrate fermentation of ammonia freeze explosion treated wheat straw by Trichoderma reesei for cellulase production. The system is based on blowing dry air through the moist substrate to cause controlled evaporative cooling, at the same time replenishing lost water by cool water spray. Solid substrate fermentation (SSF) could be an alternative to submerged fermentation (SF) for producing fuel and animal feed from agricultural residues such as wheat straw and corn stover. The main advantage of SSF, compared to SF, is the high volumetric productivity which is based on the low water content of substrate and product. This reduces fermenter volume, simplifies technology, increases product concentration and saves the cost of water removal. The most critical problem for SSF commercialization is the control of moisture and temperature. The PIs plan to design and test a completely automated and computerized system based on blowing dry air through the moist substrate to cause controlled evaporative heat removal, and at the same time replenishing lost water by cool water spray to maintain constant moisture. If the concept can be proven in this research, a realistic technical base will be available for the scale-up of SSF processes.