Multi-User Equipment: Purchase of a High Resolution Transmission Electron Microscope

多用户设备：购买高分辨率透射电子显微镜

• 批准号: 8614242
• 负责人: Martha Powell
• 金额: --
• 依托单位: Miami University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-15 至 1989-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8614242&HistoricalAwards=false
• 关键词: Multi; User; Equipment; Purchase; Resolution

This is a proposal to replace a transmission electron microscope which is worn out and limited in quality and state-of- the-art features. The new microscope requested provides greater magnification and resolution and includes features to protect samples from electron beam damage, enabling the acquisition of greater informational content from observations of cells. A goniometer stage permits structures obscured in normal orientation to be tilted and rotated until seen clearly. Whole cell or organellar three-dimensional reconstruction from thin sections is possible with the computer aided image analyzer. The instrumentation package will be used to identify features of the cell surface, developmental processes, organellar arrangements, and organellar functions valuable in systematic and cell biological considerations of algae, aquatic fungi and higher plants. Moreover, and ecological study focuses on translocation of heavy metals in mycorrhizal associations between fungi and plant roots grown in sludge. Procurement of a new, improved instrument will significantly improve the quality and quantity of research from these research programs.

这是一个建议，以取代透射电子显微镜的磨损和有限的质量和国家的最先进的特点。所要求的新型显微镜提供更大的放大倍率和分辨率，并包括保护样品免受电子束损伤的功能，从而能够从细胞观察中获得更多的信息内容。测角仪允许在正常方向上被遮挡的结构倾斜和旋转，直到看清楚为止。全细胞或细胞器的三维重建从薄切片是可能的与计算机辅助图像分析仪。该仪器包将用于识别细胞表面的特征、发育过程、细胞器排列和细胞器功能，这些在藻类、水生真菌和高等植物的系统和细胞生物学考虑中是有价值的。此外，生态学研究主要集中在污泥中生长的真菌和植物根系之间的菌根关系中重金属的转运。采购一种新的、改进的仪器将显著提高这些研究项目的研究质量和数量。