Multi-User Equipment: Purchase of Preparatory Cryoinstrumentation for Electron Microscopy

冷冻固定是保存细胞超微结构的一种优越技术。作为我们努力建立最先进的电子显微镜设施的一步，我们请求支持购买用于电子显微镜材料低温制备的三件设备。最近开发的高压速冻装置可以固定厚度达0.5 mm的组织，而不会在环境压力下对厚组织进行超高速冷冻时造成冰晶损伤。自动冷冻替代处理器便于多个冷冻固定样品，减少二次冰晶损伤组织的机会，允许方便的低温渗透树脂，并提高冷冻替代的质量和再生率。这些仪器将支持藻类、真菌、植物、昆虫和脊椎动物的系统、发育和生态研究。这些设备将用于最近建造的生物科学大楼内新组织的多用户电子显微镜设施。有一个主要的大学费用分摊部分，包括资助第二个电子显微镜技术员的职位。

Cryofixation is emerging as the superior technique for ultrastructural preservation of cells. As a step in our efforts to build-up a state-of-the-art electron microscopy facility, we request support for purchase of three items of equipment used in cryopreparation of material for electron microscopy. The recently developed high-pressure fast-freezing device allows tissues up to 0.5 mm in thickness to be fixed without the ice crystal damage accompanying ultra-rapid freezing of thick tissues at ambient pressure. The automatic freeze substitution processor facilitates multiple cryofixed samples, reduces chances of secondary ice crystal damage to tissue, allows convenient low temperature infiltration of resins, and increases quality and reproductivity of freeze-substitution. These instruments will support systematic, developmental, and ecological studies of algae, fungi, plants, insects and vertebrates. The equipment will be used in a newly organized multi-user electron microscope facility located in a recently constructed biological sciences building. There is a major university cost-sharing component, including funding of a second electron microscope technician's position.