Transport Phenomena Associated with Gas-Liquid Dispersions in Tower Fermentors for Plant and Animal Cell Culture Applications

与植物和动物细胞培养应用的塔式发酵罐中气液分散相关的传输现象

• 批准号: 8619943
• 负责人: Larry Erickson
• 金额: $ 15.9万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-15 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8619943&HistoricalAwards=false
• 关键词: Transport; Phenomena; Associated; Gas; Liquid

Narrative: Airlift reactors incorporating mammalian and plant cells have a high potential for commercial utilization to synthesize a variety of important bioproducts (proteins, etc.). Some advantages of the airlift concept include low shear rates, relatively good mixing, and a decreased risk of contamination. The single most important aspect of air lift reactors is that they provide a gentle environment for the culture and therefore, preserve the integrity of fragile cells, which in turn reduces the release of undesirables into the broth. The specific objective of the research will be to obtain fundamental knowledge about the hydrodynamic forces and to characterize turbulence in multiphase flow. This information will be used to examine effects on fragile cultures, design supports for immobilized cultures, and provide scale-up information for airlift fermentors.

叙述：纳入哺乳动物和植物细胞的气升反应器在合成各种重要生物制品（蛋白质等）方面具有很高的商业利用潜力。气举概念的一些优点包括低剪切速率，相对较好的混合，以及降低污染的风险。空气提升反应器最重要的一个方面是，它们为培养提供了一个温和的环境，因此，保持脆弱细胞的完整性，这反过来又减少了不受欢迎的物质释放到肉汤中。研究的具体目标将是获得关于流体动力的基本知识，并表征多相流中的湍流。这些信息将用于检查对脆弱培养物的影响，设计固定化培养物的支持，并为空运发酵罐提供放大信息。