Ribosomal RNA Processing: Removal of the Gap in Eukaryotic 26S-28S rRNA

核糖体 RNA 加工：真核 26S-28S rRNA 中缺口的去除

• 批准号: 8620212
• 负责人: Vassie Ware
• 金额: $ 24万
• 依托单位: Lehigh University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8620212&HistoricalAwards=false
• 关键词: Ribosomal; RNA; Processing; Removal; Gap

In general the largest mature derivative in the processing of precursor 45S ribosomal RNA (rRNA) is 26S-28S rRNA, a component of the large ribosomal subunit. Little is known about the structural elements, interacting molecules, and machinery needed to remove transcribed spacers from eukaryotic rRNA. In some eukaryotic Z6S-Z8S rRNA is further fragmented into alpha and beta halves, held together by hydrogen bonding. There is also a dearth of information about the phenomenon, which is called gap processing. The long term goals of the project are to determine the recognition signals and mechanisms involved in gap processing and to ascertain its role in ribosome function. The specific objectives of this proposal are to: 1) determine the interaction of ribosomal protein L25 with the gap region in the fungus fly Sciara coprophila by in vitro binding experiments; 2) determine the structure of rRNA in the gap region using strand-specific nucleases as probes and primer extension analysis; 3) localize the site of gap processing by subcellular fractionation of Tetrahymena; and 4) determine the fate of the excised rRNA by hybridization and S1 nuclease or Northern blot analyses. These studies should provide a better understanding of gap processing and the expression of mature rRNA in eukaroyotes.

一般最大的成熟衍生品在加工 前体45 S核糖体RNA（rRNA）的一部分是26 S-28 S rRNA， 核糖体大亚基的组成部分。 知之甚少 关于结构元素、相互作用的分子以及 需要机器来移除转录的间隔区， 真核生物rRNA。 在一些真核生物中，Z6 S-Z 8 S rRNA进一步被 分裂成α和β两半， 氢键 此外， 这种现象被称为间隙处理。 的 该项目的长期目标是确定 间隙加工中的识别信号和机制 并确定其在核糖体功能中的作用。 本建议的具体目标是：1） 确定核糖体蛋白L25与 离体条件下嗜粪黑翅菌的缺口区研究 结合实验; 2）确定rRNA的结构， 使用链特异性核酸酶作为探针的差距区域， 引物延伸分析; 3）定位缺口位点 通过四膜虫的亚细胞分级处理;和 4)通过杂交确定切除的rRNA的命运 和S1核酸酶或北方印迹分析。 这些研究 应能更好地了解差距处理， 成熟rRNA在真核生物中的表达。