Development of an Advanced Laboratory Course in Biochemical Techniques and Instrumentation

该项目将导致本科生的发展 生物化学实验室课程，强调现代技术和 仪器仪表 实验室课程将补充正在进行的 生物化学讲座课程，并将大大提高 北卡罗来纳州大学的生物化学教育 在威尔明顿。 该项目将提供一套仪器 （包括自动记录滴定系统、液体 色谱系统，密度计，制备型超浓缩凝胶， 和电泳单元）将主要用于新的 生物化学实验室课程，也在物理和分析 化学课程。 学生将获得缓冲器和其他 试剂制备、蛋白质测定、蛋白质纯化、蛋白质和 DNA电泳，酶动力学，胰蛋白酶肽消化， 膜囊泡构建、DNA分离和放射性同位素 标签。 实验工作将阐明 生物化学，并将帮助学生达到广泛使用的熟练程度 生化技术

This project will result in the development of an undergraduate biochemistry laboratory course that emphasizes modern techniques and instrumentation. The laboratory course will complement the ongoing biochemistry lecture course, and will enhance substantially the biochemistry education of students at the University of North Carolina at Wilmington. This project will provide a suite of instruments (including an automatic recording titration system, a liquid chromatography system, a densitometer, a preparative ultracentrifuge, and an electrophoresis unit) to be used primarily in the new biochemistry laboratory course and also in physical and analytical chemistry courses. Students will gain experience with buffer and other reagent preparation, protein assays, protein purification, protein and DNA electrophoresis, enzyme kinetics, tryptic peptide digestion, membrane vesicle construction, DNA isolation, and radioisotope labelling. The experimental work will illustrate important concepts in biochemistry, and will help students attain proficiency in widely used biochemical techniques.